CAJ | 학술논문

目的：探讨子痫前期患者胎盘组织及血清中转化生长因子β1（TGF-β1）表达及其意义。方法采用免疫组化SABC法和Western blot法检测30例正常晚期妊娠孕妇（对照组）和40例子痫前期患者（子痫前期组，其中轻度22例、重度18例）胎盘组织中TGF-β1的蛋白定位和表达；采用酶联免疫吸附实验的方法检测其血清TGF-β1水平。结果（1）TGF-β1蛋白主要定位于胎盘绒毛滋养细胞和蜕膜细胞，主要表达部位为细胞质。（2）轻、重度子痫前期组胎盘组织中TGF-β1的表达均高于对照组（P＜0.01），其中重度子痫前患者均高于轻度组（P＜0.05）。（3）轻、重度子痫前期患者血浆TGF-β1水平显著高于对照组（P＜0.01），其中重度显著高于轻度组（P＜0.05）。（4）子痫前期患者胎盘组织中 TGF-β1的表达与血清TGF-β1水平呈正相关（P＜0.01）。结论 TGF-β1可能参与子痫前期的发生、发展并与病情严重程度密切相关。
목적：탐토자간전기환자태반조직급혈청중전화생장인자β1（TGF-β1）표체급기의의。방법채용면역조화SABC법화Western blot법검측30례정상만기임신잉부（대조조）화40례자간전기환자（자간전기조，기중경도22례、중도18례）태반조직중TGF-β1적단백정위화표체；채용매련면역흡부실험적방법검측기혈청TGF-β1수평。결과（1）TGF-β1단백주요정위우태반융모자양세포화세막세포，주요표체부위위세포질。（2）경、중도자간전기조태반조직중TGF-β1적표체균고우대조조（P＜0.01），기중중도자간전환자균고우경도조（P＜0.05）。（3）경、중도자간전기환자혈장TGF-β1수평현저고우대조조（P＜0.01），기중중도현저고우경도조（P＜0.05）。（4）자간전기환자태반조직중 TGF-β1적표체여혈청TGF-β1수평정정상관（P＜0.01）。결론 TGF-β1가능삼여자간전기적발생、발전병여병정엄중정도밀절상관。
Objective To investigate the expression of TGF-β1 in placenta and serum from normal pregnant women and preeclampsia, so as to probe the possible mechanism of preeclampsia. Methods Placentas and serum were collected from 40 preeclampsia women including 22 mild preeclampsia and 18 severe preeclampsia as study group and 30 normal pregnant women as control group. The levels of TGF-β1 in placenta were detected by Western blot. Cellular location was determined by immunohistochemistry. The levels of TGF-β1 in serum were detected by enzyme linked immunosorbent assay. Results (1)The expressions of TGF-β1 protein was located in the trophoblast cell and also can be found in exuviate membrane organization. in placental villous syncytiontrophoblast of preeclampsia group were significantly higher than that of control group. (2)The results of Western Blot showed the expressions of TGF-β1 in severe and mild preeclampsia group were both significantly higher than that of control group(P<0.01), and was higher in severe group than that in mild one. (P<0.05). (3) The level of TGF-β1 in serum of the the mild preeclampsia group and the sever group were significantly higher than that of control group(P<0.01). and was higher in severe group than that in mild one. (P<0.05). (4) There were positively correlation between the expressions of TGF-β1 in placental and the level of TGF-β1 in serum of preeclampsia group. Conclusion TGF-β1 not only participated in pathogenic process of preeclampsia but also were related to its degree.