中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
24期
11259-11262
,共4页
晋云%江行%卿德科%罗丁
晉雲%江行%卿德科%囉丁
진운%강행%경덕과%라정
癌,肝细胞%miR-122%E-cadherin%转化生长因子β1
癌,肝細胞%miR-122%E-cadherin%轉化生長因子β1
암,간세포%miR-122%E-cadherin%전화생장인자β1
Carcinoma,hepatocellular%miR-122%E-cadherin%Transforming growth factor beta1
目的：检测肝细胞癌（HCC）组织中 miR-122和上皮性钙粘蛋白（E-cadherin）、转化生长因子-β1（TGF-β1）的表达，并分析miR-122与E-cadherin、TGF-β1之间的相关性。方法应用实时荧光定量PCR技术检测45例HCC及其配对癌旁组织中miR-122的表达情况；免疫组织化学法检测上述HCC及其配对癌旁组织中E-cadherin、TGF-β1的表达，分析miR-122的表达量与E-cadherin、TGF-β1之间的相关性。结果 miR-122在肝癌组织低表达，肝癌组织中miR-122表达量低于其癌旁组织者有41例，表达量相仿或高于其癌旁组织者4例，差异具有统计学意义（P＜0.05）。E-cadherin在HCC组织中的表达显著低于癌旁组织（P＜0.05），HCC组织中TGF-β1表达显著高于癌旁组织（P＜0.05）。HCC组织中miR-122表达与E-cadherin呈正相关（r＝0.827），与TGF-β1表达呈负相关（r＝-0.356）。结论 miR-122在HCC组织中低表达，其表达量与肝癌分化程度正相关。
目的：檢測肝細胞癌（HCC）組織中 miR-122和上皮性鈣粘蛋白（E-cadherin）、轉化生長因子-β1（TGF-β1）的錶達，併分析miR-122與E-cadherin、TGF-β1之間的相關性。方法應用實時熒光定量PCR技術檢測45例HCC及其配對癌徬組織中miR-122的錶達情況；免疫組織化學法檢測上述HCC及其配對癌徬組織中E-cadherin、TGF-β1的錶達，分析miR-122的錶達量與E-cadherin、TGF-β1之間的相關性。結果 miR-122在肝癌組織低錶達，肝癌組織中miR-122錶達量低于其癌徬組織者有41例，錶達量相倣或高于其癌徬組織者4例，差異具有統計學意義（P＜0.05）。E-cadherin在HCC組織中的錶達顯著低于癌徬組織（P＜0.05），HCC組織中TGF-β1錶達顯著高于癌徬組織（P＜0.05）。HCC組織中miR-122錶達與E-cadherin呈正相關（r＝0.827），與TGF-β1錶達呈負相關（r＝-0.356）。結論 miR-122在HCC組織中低錶達，其錶達量與肝癌分化程度正相關。
목적：검측간세포암（HCC）조직중 miR-122화상피성개점단백（E-cadherin）、전화생장인자-β1（TGF-β1）적표체，병분석miR-122여E-cadherin、TGF-β1지간적상관성。방법응용실시형광정량PCR기술검측45례HCC급기배대암방조직중miR-122적표체정황；면역조직화학법검측상술HCC급기배대암방조직중E-cadherin、TGF-β1적표체，분석miR-122적표체량여E-cadherin、TGF-β1지간적상관성。결과 miR-122재간암조직저표체，간암조직중miR-122표체량저우기암방조직자유41례，표체량상방혹고우기암방조직자4례，차이구유통계학의의（P＜0.05）。E-cadherin재HCC조직중적표체현저저우암방조직（P＜0.05），HCC조직중TGF-β1표체현저고우암방조직（P＜0.05）。HCC조직중miR-122표체여E-cadherin정정상관（r＝0.827），여TGF-β1표체정부상관（r＝-0.356）。결론 miR-122재HCC조직중저표체，기표체량여간암분화정도정상관。
Objective To detect the expression of microRNA-122 (miR-122), E-cadherin(E-cad) protein, transforming growth factor-β1(TGF-β1) protein and analyze the correlation between the expression of miR-122 and E-cad, TGF--β1 in the samples of patients with HCC. Methods The quantitative reverse transcription-PCR was used to detect the expression of miR-122 in 45 patients with HCC and the immunohistochemical method was used to detect the expression of E-cadherin and TGF-β1 protein in the tissues of HCC patients.The correlation between the miR-122 and the E-cadherin,TGF-β1 was analyzed. Results The expression of miR-122 was low in HCC and the expression of tumor tissues was lowed than side-tumor tissues in 41 patients with HCC and there were significant differences between them(P<0.05).The expression of E-cadherin was significantly lower in tumor tissues and the side-tumor tissues(P<0.05). The expression of TGF-β1 was significantly higher in tumor tissues and the side-tumor tissues(P<0.05). It showed positive correlation between the miR-122 expression and the E-cadherin expression and it showed negative correlation between the miR-122 expression and the TGF-β1 expression. Conclusion The expression of miR-122 in the patients with HCC was low and correlated positively with the tumor differentiation of HCC.
