CAJ | 학술논문

目的：慢性高原病（CMS）是高海拔地区常见病。本文通过研究CMS患者骨髓单个核细胞（BMMNCs）凋亡及Fas、FasL、TNFR和TNF表达情况，探讨细胞凋亡及凋亡分子在CMS发病中的作用。方法选取CMS患者35例，采用电镜定性观察骨髓造血细胞凋亡情况，TUNEL技术定量研究BMMNCs凋亡指数，流式细胞术检测BMMNCs凋亡分子Fas、FasL、TNFR及TNF表达水平，并与健康对照组（20例）比较。结果（1）CMS患者骨髓造血细胞存在凋亡现象，CMS患者BMMNCs凋亡指数明显低于对照组，分别为（10.13±9.80）%及（21.32±11.97）%（P＜0.01）；（2）BMMNCs凋亡分子Fas、FasL、TNFR及TNF表达水平在两组间无统计学差异。结论 CMS患者BMMNCs凋亡下调，可能在CMS红细胞积累中发挥一定作用，但其凋亡异常的机制比较复杂，可能涉及多种凋亡信号转导途径。
목적：만성고원병（CMS）시고해발지구상견병。본문통과연구CMS환자골수단개핵세포（BMMNCs）조망급Fas、FasL、TNFR화TNF표체정황，탐토세포조망급조망분자재CMS발병중적작용。방법선취CMS환자35례，채용전경정성관찰골수조혈세포조망정황，TUNEL기술정량연구BMMNCs조망지수，류식세포술검측BMMNCs조망분자Fas、FasL、TNFR급TNF표체수평，병여건강대조조（20례）비교。결과（1）CMS환자골수조혈세포존재조망현상，CMS환자BMMNCs조망지수명현저우대조조，분별위（10.13±9.80）%급（21.32±11.97）%（P＜0.01）；（2）BMMNCs조망분자Fas、FasL、TNFR급TNF표체수평재량조간무통계학차이。결론 CMS환자BMMNCs조망하조，가능재CMS홍세포적루중발휘일정작용，단기조망이상적궤제비교복잡，가능섭급다충조망신호전도도경。
Objective To observe the expressions of Fas, FasL, TNFR and TNF and the change of apoptosis of bone marrow mononuclear cells (BMMNCs) in patients with chronic mountain sickness(CMS). Methods The bone marrow specimens were collected from 35 CMS patients and 20 healthy adults and the BMMNCs were separated. Apoptosis was observed qualitatively by electron microscopy. The apoptotic index (AI) of BMMNCs was measured by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique. Flow cytometry was used to detect expressions of Fas, FasL, TNFR and TNF of BMMNCs. Results (1)There was apoptosis of BMMNCs in patients with CMS. The AI of BMMNCs in CMS patients was lower than that in the controls (10.13%±9.80 % vs. 21.32%±11.97%, P<0.01). (2) The significant difference was not found between the two groups in the expression of Fas, FasL, TNFR or TNF. Conclusions The results showed that apoptosis of BMMNCs was down-regulated in CMS patients, which may play a role in the accumulation of red blood cells. The mechanism of change of hematopoietic cells apoptosis in CMS patients is complicated, other apoptotic signal transduction pathways may be involved in it.