CAJ | 학술논문

目的：观察银杏叶提取物（extract of ginkgo bilobaleaf ，EGB761）在高糖高胰岛素作用下对视网膜Müller细胞活性的改变及其保护作用。方法用30mmol/L葡萄糖和（或）200U/L的胰岛素处理大鼠视网膜Müller细胞48小时，分为高糖高胰岛素组：200U/L胰岛素＋30mmol/L葡萄糖；高胰岛素组：200U/L 胰岛素；对照组：普通低糖型DMEM培养（含葡萄糖5mmol/L ）。提前0.5小时加入60μg/mL、120μg/mL的EGB761，观察对各组细胞的保护作用，48小时后用MTT 法测定视网膜Müller细胞的活性。结果高糖高浓度胰岛素处理后Müller细胞活性降低，与对照组相比差异有统计学意义（ P＜0.05）。而60或120μg/mL的EGB761能上调高浓度葡萄糖和高胰岛素处理后的视网膜Müller细胞的活力，且在单纯高胰岛素组中更佳，与无EGB761处理组相比差异有统计学意义（ P＜0.05）。结论高浓度葡萄糖和高胰岛素在短期内可使视网膜Müller细胞的活性降低，一定浓度的EGB761可减轻高糖高胰岛素对视网膜Müller细胞的损伤。
목적：관찰은행협제취물（extract of ginkgo bilobaleaf ，EGB761）재고당고이도소작용하대시망막Müller세포활성적개변급기보호작용。방법용30mmol/L포도당화（혹）200U/L적이도소처리대서시망막Müller세포48소시，분위고당고이도소조：200U/L이도소＋30mmol/L포도당；고이도소조：200U/L 이도소；대조조：보통저당형DMEM배양（함포도당5mmol/L ）。제전0.5소시가입60μg/mL、120μg/mL적EGB761，관찰대각조세포적보호작용，48소시후용MTT 법측정시망막Müller세포적활성。결과고당고농도이도소처리후Müller세포활성강저，여대조조상비차이유통계학의의（ P＜0.05）。이60혹120μg/mL적EGB761능상조고농도포도당화고이도소처리후적시망막Müller세포적활력，차재단순고이도소조중경가，여무EGB761처리조상비차이유통계학의의（ P＜0.05）。결론고농도포도당화고이도소재단기내가사시망막Müller세포적활성강저，일정농도적EGB761가감경고당고이도소대시망막Müller세포적손상。
Objective To investigate the activity of cultured retinal Müller cell under high glucose and high insulin condition ,and study the effects of EGB761 Müller cells’ activity .Methods Müller cells from rat retina were collected and cultured in vitro .They were treated with glucose 30 mmol/L and(or) insulin 200U/L for 48 hours .They were divided into three groups :high glucose and high insulin group with insulin 200U/L and glucose 30mmol/L;high insulin and low glucose group with insulin 200U/L and glucose 5mmol/L;control group with glucose 5mmol/L and no insulin .Different concentrations of EGB761 were added to the medium in all groups and investigated the effects of EGB761 Müller cells’ activity .There were three concentrations :0μg/mL ,60μg/mL ,120μg/mL .After 48 hours incubation ,the ac-tivity of Müller cells evaluated by MTT . Results After 200U/L insulin and glucose 30mmol/Ltreatment , the activity of Müller cells de-creased dramatically as compared with that of control group ( P<0.05) .After EGB761 treatment in high insulin and high glucose group ,the activity of Müller cells were increased compared with those without EGB761 treatment ( P<0.05) .Conclusion High concentration of glu-cose and high insulin could decrease the activity of Müller cells in a short time .After treatment of EGB761 ,the activity of Müller cells could recover .