中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
1期
9-11
,共3页
张天丹%杨曼曼%杨璐全%邹永林%傅俊江
張天丹%楊曼曼%楊璐全%鄒永林%傅俊江
장천단%양만만%양로전%추영림%부준강
D1S80位点%VNTR%遗传多态性%川南地区
D1S80位點%VNTR%遺傳多態性%川南地區
D1S80위점%VNTR%유전다태성%천남지구
D1S80 locus%VNTR%Genetic polymorphism%Southern Sichuan
目的:了解川南地区汉族人群D1S80位点群体遗传多态性。方法采用PCR结合聚丙烯酰胺PAGE电泳及高灵敏度银染技术,即Amp-FLP方法对川南地区120名汉族无血缘关系个体D1S80位点进行多态性分析。结果在所调查的120名无关个体样本中,观察到D1S80基因座有18个等位基因,基因频率分布在0.004166667~0.1958333333之间,杂合度为0.7869,个体识别力为0.87,非父排除率为0.8152,其基因型频率分布符合Hardy-Weinberg平衡法则。结论D1S80基因座的PCR分型具有较好的准确性和灵敏度,为法医学个体识别和亲权鉴定、遗传病基因链锁分析等的研究及应用提供了川南地区有用的遗传信息。
目的:瞭解川南地區漢族人群D1S80位點群體遺傳多態性。方法採用PCR結閤聚丙烯酰胺PAGE電泳及高靈敏度銀染技術,即Amp-FLP方法對川南地區120名漢族無血緣關繫箇體D1S80位點進行多態性分析。結果在所調查的120名無關箇體樣本中,觀察到D1S80基因座有18箇等位基因,基因頻率分佈在0.004166667~0.1958333333之間,雜閤度為0.7869,箇體識彆力為0.87,非父排除率為0.8152,其基因型頻率分佈符閤Hardy-Weinberg平衡法則。結論D1S80基因座的PCR分型具有較好的準確性和靈敏度,為法醫學箇體識彆和親權鑒定、遺傳病基因鏈鎖分析等的研究及應用提供瞭川南地區有用的遺傳信息。
목적:료해천남지구한족인군D1S80위점군체유전다태성。방법채용PCR결합취병희선알PAGE전영급고령민도은염기술,즉Amp-FLP방법대천남지구120명한족무혈연관계개체D1S80위점진행다태성분석。결과재소조사적120명무관개체양본중,관찰도D1S80기인좌유18개등위기인,기인빈솔분포재0.004166667~0.1958333333지간,잡합도위0.7869,개체식별력위0.87,비부배제솔위0.8152,기기인형빈솔분포부합Hardy-Weinberg평형법칙。결론D1S80기인좌적PCR분형구유교호적준학성화령민도,위법의학개체식별화친권감정、유전병기인련쇄분석등적연구급응용제공료천남지구유용적유전신식。
Objective To study the population genetic polymorphism of D1S80 locus in Han population in Southern Sichuan. Methods PCR amplification, electrophoresis of PAGE and high sensitive silver staining method, namely Amp-FLP, were performed for D1S80 loci by polymorphic analysis in 120 unrelated Han individuals from Southern Sichuan. Results From survey of 120 unrelated individuals for D1S80 loci, 18 alleles are observed. Gene frequency is located between 0.004166667 and 0.1958333333,heterozygosity is 0.7869, discrimination power is 0.87,exclusion power is 0.8152,which is in accordance with Hardy-Weinberg balance rule for the genotype distribution. Conclusion D1S80 gene loci PCR typing has good accuracy and sensitivity. It provides useful genetic information for forensic science research on individual identification and paternity test,genetic disease gene linkage analysis for D1S80 gene loci from Southern Sichuan.