CAJ | 학술논문

目的：了解川南地区汉族人群D1S80位点群体遗传多态性。方法采用PCR结合聚丙烯酰胺PAGE电泳及高灵敏度银染技术，即Amp-FLP方法对川南地区120名汉族无血缘关系个体D1S80位点进行多态性分析。结果在所调查的120名无关个体样本中，观察到D1S80基因座有18个等位基因，基因频率分布在0.004166667～0.1958333333之间，杂合度为0.7869，个体识别力为0.87，非父排除率为0.8152，其基因型频率分布符合Hardy-Weinberg平衡法则。结论D1S80基因座的PCR分型具有较好的准确性和灵敏度，为法医学个体识别和亲权鉴定、遗传病基因链锁分析等的研究及应用提供了川南地区有用的遗传信息。
목적：료해천남지구한족인군D1S80위점군체유전다태성。방법채용PCR결합취병희선알PAGE전영급고령민도은염기술，즉Amp-FLP방법대천남지구120명한족무혈연관계개체D1S80위점진행다태성분석。결과재소조사적120명무관개체양본중，관찰도D1S80기인좌유18개등위기인，기인빈솔분포재0.004166667～0.1958333333지간，잡합도위0.7869，개체식별력위0.87，비부배제솔위0.8152，기기인형빈솔분포부합Hardy-Weinberg평형법칙。결론D1S80기인좌적PCR분형구유교호적준학성화령민도，위법의학개체식별화친권감정、유전병기인련쇄분석등적연구급응용제공료천남지구유용적유전신식。
Objective To study the population genetic polymorphism of D1S80 locus in Han population in Southern Sichuan. Methods PCR amplification, electrophoresis of PAGE and high sensitive silver staining method, namely Amp-FLP, were performed for D1S80 loci by polymorphic analysis in 120 unrelated Han individuals from Southern Sichuan. Results From survey of 120 unrelated individuals for D1S80 loci, 18 alleles are observed. Gene frequency is located between 0.004166667 and 0.1958333333,heterozygosity is 0.7869, discrimination power is 0.87,exclusion power is 0.8152,which is in accordance with Hardy-Weinberg balance rule for the genotype distribution. Conclusion D1S80 gene loci PCR typing has good accuracy and sensitivity. It provides useful genetic information for forensic science research on individual identification and paternity test,genetic disease gene linkage analysis for D1S80 gene loci from Southern Sichuan.