CAJ | 학술논문

通过根系差异蛋白质组学探讨了种植方式（复种连作、复种轮作）对烤烟生长的影响。结果表明，共有15个蛋白质表达丰度发生变化，用 LC-MS/MS 鉴定了14个差异蛋白质点，经生物信息学分析，11个蛋白质的功能有注释，其中3个属于与香气形成有关的蛋白质，包括转酮醇酶、单脱氢抗坏血还原酶和 O-甲基转移酶，它们在复种轮作条件下均上调表达，此可能有助于烟叶品质提高，而复种连作处理下调表达，可能不利于品质提高。还鉴定到涉及能量、抗性、物质运输、核酸等功能的蛋白：磷酸甘油酸酯激酶、线粒体内膜移位酶亚基 Tim13、NBS-LRR、DnaJ、细胞内囊泡运输蛋白 Sly1、核糖体蛋白质，在复种轮作下也上调表达，此可能促进烤烟的生长，有利于提高烟草产量和品质且经济效益较好。而在复种连作处理下调表达，进而可能使得烟草生长变弱、产量降低、品质变差。
통과근계차이단백질조학탐토료충식방식（복충련작、복충륜작）대고연생장적영향。결과표명，공유15개단백질표체봉도발생변화，용 LC-MS/MS 감정료14개차이단백질점，경생물신식학분석，11개단백질적공능유주석，기중3개속우여향기형성유관적단백질，포괄전동순매、단탈경항배혈환원매화 O-갑기전이매，타문재복충륜작조건하균상조표체，차가능유조우연협품질제고，이복충련작처리하조표체，가능불리우품질제고。환감정도섭급능량、항성、물질운수、핵산등공능적단백：린산감유산지격매、선립체내막이위매아기 Tim13、NBS-LRR、DnaJ、세포내낭포운수단백 Sly1、핵당체단백질，재복충륜작하야상조표체，차가능촉진고연적생장，유리우제고연초산량화품질차경제효익교호。이재복충련작처리하조표체，진이가능사득연초생장변약、산량강저、품질변차。
Root differential proteomics was employed in this study to explore the impact of different planting patterns (multiple cropping rotation (MR) and consecutive monoculture multiple cropping (MC) modes) on growth of flue-cured tobacco (Nicotina tobaccum L). The results showed that a total of 15 protein spots had significant expression difference; 14 protein spots were identified by LC-MS/MS analysis and database searching. Among which, the function of 11 proteins were identified, three proteins were related to the aroma formation, i.e. transketolase, monodehydroascorbate reductase(MDHA), O-methyltransferase-like protein were up-regulated under MR, it was helpful probably to enhance the tobacco quality. While under the MC treatment, they were down-regulated, and the tobacco quality could be worse. Phosphoglycerate kinase, mitochondrial import inner membrane translocase subunit Tim13, NBS-LRR(nucleotide binding site plus leucine-rich repeat), DnaJ protein, Vesicle trafficking protein Sly1, Ribosomal protein-like protein were involved in energy metabolism, plant antioxidation reaction, translocation of solute, metabolism of nucleic acid, respectively. They were very important for the growth development of tobacco and all up-regulated under MR treatments, probably increasing the yield and improving the quality of tobacco, and gaining better economic benefit. They were all down-regulated under MC, which may cause weak growth, low yield and poor quality of tobacco.