CAJ | 학술논문

为寻求一种快速高通量筛选鉴定烟草抗赤星病种质或材料的方法，本研究以烟草（Nicotiana tobacum L.）种质净叶黄、Beinhart1000-1、NC89和G140作为供试材料，利用烟草赤星病菌毒素就烟草种质对赤星病的抗病性鉴定方法进行研究，试验设计3种鉴定方法：毒素液浸根法、毒素液浸种法和毒素培养基法。为验证毒素培养基法鉴定结果的可靠性，对烟草种质材料净叶黄、Beinhart1000-1、NC89、G140、K326、G28和中烟100的室内鉴定结果，与其对烟草赤星病的田间抗性鉴定结果进行了相关性分析。结果表明，室内鉴定结果与田间鉴定结果之间极显著相关（r=-0.9588），室内鉴定结果可准确反映烟草种质对赤星病的抗病性。
위심구일충쾌속고통량사선감정연초항적성병충질혹재료적방법，본연구이연초（Nicotiana tobacum L.）충질정협황、Beinhart1000-1、NC89화G140작위공시재료，이용연초적성병균독소취연초충질대적성병적항병성감정방법진행연구，시험설계3충감정방법：독소액침근법、독소액침충법화독소배양기법。위험증독소배양기법감정결과적가고성，대연초충질재료정협황、Beinhart1000-1、NC89、G140、K326、G28화중연100적실내감정결과，여기대연초적성병적전간항성감정결과진행료상관성분석。결과표명，실내감정결과여전간감정결과지간겁현저상관（r=-0.9588），실내감정결과가준학반영연초충질대적성병적항병성。
In order to find out an approach to rapidly identify resistant tobacco germplasm to Alternaria alternata, tobacco varieties Jingyehuang, Beinhart1000-1, NC89 and G140 were used as test materials. Using toxins produced by brown spot fungus (Alternaria alternata), we carried out a comparative test to choose a better screening method. Three kinds of screening methods were designed:toxins liquid soaking root of tobacco, toxins liquid soaking seeds of tobacco, and toxin-containing medium screening method. To verify the reliability of the toxin-containing medium screening method, Correlation between laboratory test results and field data on tobacco varieties Jingyehuang, Beinhart1000-1, NC89, G140, K326, G28 and Zhongyan100 was analyzed, and correlation coefficient of-0.9588 was found, which suggests the effectiveness of laboratory method is close to those of field.