CAJ | 학술논문

通过质外体提取和测定氮素代谢相关酶等方法，调查了不同浓度谷氨酰胺合成酶（GS）抑制剂（Glufosinate）对2个氮效率烤烟品种氮代谢相关生理指标的影响。结果表明，GS抑制剂可以有效抑制衰老期间烟叶GS活性，使氮素再同化能力下降，促进氮素营养物质的降解，并诱导谷氨酸脱氢酶（GDH）活性升高和硝酸还原酶（NR）活性下降，使叶片NH4+积累，增大了氨气挥发潜力，且随着GS抑制剂浓度的升高，抑制效果更加明显。氮低效品种与氮高效品种相比，衰老速度快，叶片NH4+的积累量小，氨气挥发潜力大，品种间差异显著。GS抑制剂可以加速叶片衰老，促进氮素降解和再转移。
통과질외체제취화측정담소대사상관매등방법，조사료불동농도곡안선알합성매（GS）억제제（Glufosinate）대2개담효솔고연품충담대사상관생리지표적영향。결과표명，GS억제제가이유효억제쇠로기간연협GS활성，사담소재동화능력하강，촉진담소영양물질적강해，병유도곡안산탈경매（GDH）활성승고화초산환원매（NR）활성하강，사협편NH4+적루，증대료안기휘발잠력，차수착GS억제제농도적승고，억제효과경가명현。담저효품충여담고효품충상비，쇠로속도쾌，협편NH4+적적루량소，안기휘발잠력대，품충간차이현저。GS억제제가이가속협편쇠로，촉진담소강해화재전이。
By using the method of apoplast extraction and determination of enzymes related nitrogen metabolism, the influence of Glufosinate (GS) inhibitor concentrations on nitrogen metabolism physiological indices of flue-cured tobacco varieties with two nitrogen use efficiency were studied. The results show that GS inhibitors could inhibit GS activity of tobacco leaf during senescence, reduce the ability of nitrogen assimilation, promote the degradation of nitrogen nutrient, induct the increasing of glutamate dehydrogenase (GDH) activity and decreasing of nitrate reductase (NR) activity and promote accumulation of leaf NH4+, increase ammonia volatilization potential. These inhibition effects became more obvious with increasing GS inhibitor concentration. Compared with high nitrogen efficiency varieties, low nitrogen efficiency varieties aged more rapidly and had a lower NH4+accumulation and ammonia volatilization potential. There was significant difference between varieties. This shows that GS inhibitors can accelerate leaf senescence and promote nitrogen degradation and transfer.