中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
2期
178-181
,共4页
李存香%魏柏青%熊浩明%祁美英%杨晓艳%辛有全%魏荣杰%靳娟%代瑞霞
李存香%魏柏青%熊浩明%祁美英%楊曉豔%辛有全%魏榮傑%靳娟%代瑞霞
리존향%위백청%웅호명%기미영%양효염%신유전%위영걸%근연%대서하
鼠疫%差异区段基因分型技术%溯源
鼠疫%差異區段基因分型技術%溯源
서역%차이구단기인분형기술%소원
Plague%Different region molecular typing techniques%Infection source
目的 对1958-2012年青海省人间鼠疫流行期间自鼠疫患者和尸体中分离的119株鼠疫菌进行病原学研究.方法 采用常规方法和分子生物学技术对鼠疫菌进行病原学研究,同时采用差异区段(DFR)基因分型技术对2004年囊谦县、2009年兴海县人间肺鼠疫暴发疫情进行溯源分析.结果 119株鼠疫菌中,105株属青藏高原型菌株,6株属祁连山型菌株,8株菌具有特殊生化特性;84.03％(100/119)的鼠疫菌具有4个毒力因子(F1+、Pst Ⅰ+、VW+、pgm+).测试的74株菌中,72株(97.30％)为强毒菌;携带大质粒52×106、65×106、92×106的菌株主要分布于海南、海北、海西、玉树、果洛、黄南6个州和湟源县;鼠疫菌DFR基因型以5、8型为主.其中5型占44.54％(53/119),分布于都兰、湟源、玉树、杂多、治多、称多、曲麻莱、玛多、囊谦、祁连等地区;8型占32.77％(39/119),分布于祁连山南北麓、青海湖环湖地区.2004年囊谦县肺鼠疫暴发分离株均为10型;2009年兴海县肺鼠疫暴发分离株(来自鼠疫患者、人尸和牧犬体内)的基因型均为8型.结论 本次试验菌株均具备青藏高原鼠疫病原体特性.菌株溯源分析显示,基于DFR的鼠疫菌基因分型与流行病学调查一致,可用于确定传染源.
目的 對1958-2012年青海省人間鼠疫流行期間自鼠疫患者和尸體中分離的119株鼠疫菌進行病原學研究.方法 採用常規方法和分子生物學技術對鼠疫菌進行病原學研究,同時採用差異區段(DFR)基因分型技術對2004年囊謙縣、2009年興海縣人間肺鼠疫暴髮疫情進行溯源分析.結果 119株鼠疫菌中,105株屬青藏高原型菌株,6株屬祁連山型菌株,8株菌具有特殊生化特性;84.03％(100/119)的鼠疫菌具有4箇毒力因子(F1+、Pst Ⅰ+、VW+、pgm+).測試的74株菌中,72株(97.30％)為彊毒菌;攜帶大質粒52×106、65×106、92×106的菌株主要分佈于海南、海北、海西、玉樹、果洛、黃南6箇州和湟源縣;鼠疫菌DFR基因型以5、8型為主.其中5型佔44.54％(53/119),分佈于都蘭、湟源、玉樹、雜多、治多、稱多、麯痳萊、瑪多、囊謙、祁連等地區;8型佔32.77％(39/119),分佈于祁連山南北麓、青海湖環湖地區.2004年囊謙縣肺鼠疫暴髮分離株均為10型;2009年興海縣肺鼠疫暴髮分離株(來自鼠疫患者、人尸和牧犬體內)的基因型均為8型.結論 本次試驗菌株均具備青藏高原鼠疫病原體特性.菌株溯源分析顯示,基于DFR的鼠疫菌基因分型與流行病學調查一緻,可用于確定傳染源.
목적 대1958-2012년청해성인간서역류행기간자서역환자화시체중분리적119주서역균진행병원학연구.방법 채용상규방법화분자생물학기술대서역균진행병원학연구,동시채용차이구단(DFR)기인분형기술대2004년낭겸현、2009년흥해현인간폐서역폭발역정진행소원분석.결과 119주서역균중,105주속청장고원형균주,6주속기련산형균주,8주균구유특수생화특성;84.03％(100/119)적서역균구유4개독력인자(F1+、Pst Ⅰ+、VW+、pgm+).측시적74주균중,72주(97.30％)위강독균;휴대대질립52×106、65×106、92×106적균주주요분포우해남、해북、해서、옥수、과락、황남6개주화황원현;서역균DFR기인형이5、8형위주.기중5형점44.54％(53/119),분포우도란、황원、옥수、잡다、치다、칭다、곡마래、마다、낭겸、기련등지구;8형점32.77％(39/119),분포우기련산남북록、청해호배호지구.2004년낭겸현폐서역폭발분리주균위10형;2009년흥해현폐서역폭발분리주(래자서역환자、인시화목견체내)적기인형균위8형.결론 본차시험균주균구비청장고원서역병원체특성.균주소원분석현시,기우DFR적서역균기인분형여류행병학조사일치,가용우학정전염원.
Objective To study the biological and genetic characteristics of 119 strains of Yersinia (Y.)pestis isolated from plague patients in Qinghai province,from 1958-2012.Methods Both regular methods and different region (DFR) molecular typing techniques were used to study the epidemiological characteristics on 119 strains of Y.pestisin Qinghai during 1958-2012.Sources of Y.pestis from two outbreaks,in Nangqian county in 2004 and in Xinghai county in 2009,Qinghai province were also analyzed.Results 105 strains of Y.pestis were identified as Qinghai-Tibet Plateau Ecotype while the other 6 strains as Qilian Mountains Ecotype.84.03％ (100/119) of the tested strains carried 4 virulence factors F 1 +,Pst Ⅰ +,VW+ and Pgm+).97.30％ (72/74) of the tested strains showed high virulence.Strains that carrying 52 × 106,65 × 106,92 × 106 plasmids were distributed in Hainan,Haibei,Haixi,Yushu,Guoluo,Huangnan and Huangyuan counties.Genomovar 5 and 8 were the main genetypes that circling around Qinghai Lake.Genomovar 10 was found in strains of Y.pestisin Nangqian county while Genomovar 8 was found in the strains isolated from human plague patient during the epidemics in Xinghai county in Qinghai.Conclusion Data from biological and genetic analyses on the epidemics of human plague in Nangqian county in 2004 and in Xinghai county in 2009 demonstrated that methods as DFR genotyping and virulence factors profiles,as well as plasmids profiles were powerful tools in confirming the human plague epidemics and sources of infection.
