CAJ | 학술논문

目的：明确转录因子E2F1在膀胱癌T24恶性生物学行为形成中的作用。方法运用siRNA干扰T24中的E2F1基因，MTT法检测沉默前后T24存活能力的变化，平板克隆形成实验检测沉默前后T24克隆形成能力的变化，Transwell小室迁移和侵袭实验检测T24迁移和侵袭能力的变化。结果 siRNA可以下降E2F187％左右的蛋白表达，E2F1被干扰后，T24的存活能力下降，克隆形成能力减弱，迁移和侵袭能力减弱，差异有统计学意义（P＜0．05）。结论siRNA沉默E2F1可以降低膀胱癌T24的恶性，提示E2F1可以作为膀胱癌一个潜在的治疗靶点。
목적：명학전록인자E2F1재방광암T24악성생물학행위형성중적작용。방법운용siRNA간우T24중적E2F1기인，MTT법검측침묵전후T24존활능력적변화，평판극륭형성실험검측침묵전후T24극륭형성능력적변화，Transwell소실천이화침습실험검측T24천이화침습능력적변화。결과 siRNA가이하강E2F187％좌우적단백표체，E2F1피간우후，T24적존활능력하강，극륭형성능력감약，천이화침습능력감약，차이유통계학의의（P＜0．05）。결론siRNA침묵E2F1가이강저방광암T24적악성，제시E2F1가이작위방광암일개잠재적치료파점。
Objective To explore the action of transcription factor E2F1 in carcinogenesis of bladder cancer T24.Mteh ods siRNA is utillized to silence E2F1 in T24.MTT is used to test viability, plate colony formation assay is used to test colony formation ability ,transwell small chamber assay is used to test migration and invasion ability comparing before and after E2F1 is silenced .Resu lts After E2F 1is silenced , 87% E2F1 protein expression is suppressed .As a result, viability, colony formation ability, migration and invasion ability is significantly inhibited.ConclusionSuppressing E2F1 can decrease malignant characteristic of T24 suggesting E2F1 can be regarded as a potential therapy target in bladder cancer .