CAJ | 학술논문

用1000 m L量筒进行全雌牙鲆卵浮性试验，每次取卵500粒，观察了全雌牙鲆卵在不同盐度下的悬浮状况。在不充气、不投饵、水温15～16℃条件下，取盐度试验孵化出的正常仔鱼100尾放入20 cm ×20 cm ×20 cm的小方型水槽中，设置10个盐度梯度，10、15、20、25、30、35、40、45、50，自然海水作对照；同时在不充气、不投饵、水温17～18℃条件下，取在自然海水中孵化出的正常仔鱼100尾放入20 cm ×20 cm ×20 cm的小方型水槽中，设置12个盐度梯度，5、10、15、20、25、30、35、40、45、50、55，自然海水作对照，观察不同盐度对全雌牙鲆胚胎发育及仔鱼存活的影响。试验结果表明，全雌牙鲆卵在盐度≤27时，全部沉底，盐度为27～30时，呈悬浮状态，盐度≥30时，全部上浮。胚胎发育在盐度为30～50时，孵化率均超过71．8％，差异不显著（ P＞0．05），畸形率小于4．3％；盐度为10～30时的孵化率超过64．5％，畸形率小于5．5％。盐度为10～25时的孵化率低于盐度30～50的孵化率，差异显著（ P＜0．05）；其畸形率高于盐度30～50的畸形率，差异不显著（ P＞0．05）。因此，高盐度比低盐度更适合胚胎发育。仔鱼在盐度为10～40时，均能很好地成活，盐度为20时，仔鱼的无投饵存活系数生存活力指数值最高。盐度5以下、50以上，仔鱼的无投饵存活系数小于5，仔鱼基本不能存活。
용1000 m L량통진행전자아평란부성시험，매차취란500립，관찰료전자아평란재불동염도하적현부상황。재불충기、불투이、수온15～16℃조건하，취염도시험부화출적정상자어100미방입20 cm ×20 cm ×20 cm적소방형수조중，설치10개염도제도，10、15、20、25、30、35、40、45、50，자연해수작대조；동시재불충기、불투이、수온17～18℃조건하，취재자연해수중부화출적정상자어100미방입20 cm ×20 cm ×20 cm적소방형수조중，설치12개염도제도，5、10、15、20、25、30、35、40、45、50、55，자연해수작대조，관찰불동염도대전자아평배태발육급자어존활적영향。시험결과표명，전자아평란재염도≤27시，전부침저，염도위27～30시，정현부상태，염도≥30시，전부상부。배태발육재염도위30～50시，부화솔균초과71．8％，차이불현저（ P＞0．05），기형솔소우4．3％；염도위10～30시적부화솔초과64．5％，기형솔소우5．5％。염도위10～25시적부화솔저우염도30～50적부화솔，차이현저（ P＜0．05）；기기형솔고우염도30～50적기형솔，차이불현저（ P＞0．05）。인차，고염도비저염도경괄합배태발육。자어재염도위10～40시，균능흔호지성활，염도위20시，자어적무투이존활계수생존활력지수치최고。염도5이하、50이상，자어적무투이존활계수소우5，자어기본불능존활。
The sinking and buoyancy of eggs were observed in hologynic Japanese flounder Paralichthys oliv aceus under various salinities to investigate the effects of salinity on embryonic development and larval survival .The results showed that hologynic Japanese flounder eggs sank at a salinity of below 27 ,were pelagic at salinity from 27 to 30 ,and were buoyancy on the water surface at a salinity of above 30 .The hatching rate of over 71 .8% was observed at a salinity of 30-50 ,no significant difference (P>0 .05) ,and deformity rate of less than 4 .3% .The hatching rate of over 64 .5% was found at salinity from 10 to 30 , with deformity rate of less than 5 .5% .There was significantly lower hatching rate at salinity from 10 to 25 than that in salinity from 30 to 50 (P <0 .05) ,while there was higher deformity at salinity from 10 to 25 than that in salinity from 30 to 50 ( P > 0 .05 ) ,indicating properly high salinity more suitable for embryonic development .The larvae tolerated the salinity from 10 to 40 ,the maximal survival activity index (SAI) value of unfed larvae at a salinity of 20 .The unfed larvae had SAI value of less than 5 (mortality ) at salinity of below 5 and over 50 .