CAJ | 학술논문

本试验分别通过体外和体内试验比较了酵母β-葡聚糖与杆菌肽锌对大肠杆菌数量的影响。结果显示：体外试验中，当培养基基础pH为6和7时，单独添加酵母β-葡聚糖培养3 h后大肠杆菌数仍显著低于对照组（P＜0.05），当基础pH为8时，培养6 h后培养基中大肠杆菌数仍显著少于对照组（P＜0.05）；体内试验中，口服大肠杆菌（O141︰K99）攻毒后，添加酵母β-葡聚糖与杆菌肽锌组ADG比对照组分别提高了30.38%和30.81%（P＜0.05），各处理组F/G均显著优于对照组（P＜0.05），同时，各处理组犊牛粪便指数显著低于对照组（P＜0.05），各处理组犊牛攻毒后12 h和24 h时直肠中大肠杆菌数量显著降低（P＜0.05）。试验结果表明，在日粮中添加75 mg/kg酵母β-葡聚糖能缓解由大肠杆菌K99攻毒所导致的生长性能下降，并能在一定程度上替代或减少抗生素的使用。
본시험분별통과체외화체내시험비교료효모β-포취당여간균태자대대장간균수량적영향。결과현시：체외시험중，당배양기기출pH위6화7시，단독첨가효모β-포취당배양3 h후대장간균수잉현저저우대조조（P＜0.05），당기출pH위8시，배양6 h후배양기중대장간균수잉현저소우대조조（P＜0.05）；체내시험중，구복대장간균（O141︰K99）공독후，첨가효모β-포취당여간균태자조ADG비대조조분별제고료30.38%화30.81%（P＜0.05），각처리조F/G균현저우우대조조（P＜0.05），동시，각처리조독우분편지수현저저우대조조（P＜0.05），각처리조독우공독후12 h화24 h시직장중대장간균수량현저강저（P＜0.05）。시험결과표명，재일량중첨가75 mg/kg효모β-포취당능완해유대장간균K99공독소도치적생장성능하강，병능재일정정도상체대혹감소항생소적사용。
The object of this experiment was to study the effect of yeast β-glucan and bacitra-cin zinc on the count of Escherichia coli in vitro and in vivo. The results showed that the number of Escherichia coli after 3 h was significant lower in the group which supplemented with yeastβ-glucan when the pH of medium were 6 and 7(P<0.05). When the pH of medium were 8, the number of Escherichia coli was significant lower in the group which supplemented with yeast β-glucan than the control group after 6 h(P<0.05). Compared with the control group, ADG of the calves in two treatments that supplemented with yeast β-glucan and bacitracin zinc were increased by 30.4 and 30.8% after the Escherichia coli challenged(P<0.05). F/G in two treatments was lower than that in the control group. The count of Escherichia coli in the rectum was decreased(P<0.05) after the challenge in 12 h and 24 h. It could be concluded that yeast β-glucan at the dosage of 75 mg/kg could erase the decrease of growth performance of pre-ruminant calves which challenged with Esche-richia coli K99, thus using β-glucan in calves feed may decreased the usage of antibiotics.