CAJ | 학술논문

目的：检测标本保存时间和保存温度，为流式自然杀伤(natural killer，NK)细胞和B细胞检测标准化操作提供科学依据。方法采用四色流式细胞术分析健康体检人群外周血NK细胞和B细胞数量百分比，检测保存时间与保存温度对实验结果的影响。结果未经处理的全血标本常温(18℃)保存后检测结果稳定，低温、高温及长时间保存都引起检测结果降低；全血标本经荧光抗体染色后，用多聚甲醛固定可在4℃条件下保存1周。结论流式细胞分析法检测NK细胞和B细胞，若标本不能及时处理宜18℃保存并在96 h内检测；处理后的样本可在4℃条件下保存1周。
목적：검측표본보존시간화보존온도，위류식자연살상(natural killer，NK)세포화B세포검측표준화조작제공과학의거。방법채용사색류식세포술분석건강체검인군외주혈NK세포화B세포수량백분비，검측보존시간여보존온도대실험결과적영향。결과미경처리적전혈표본상온(18℃)보존후검측결과은정，저온、고온급장시간보존도인기검측결과강저；전혈표본경형광항체염색후，용다취갑철고정가재4℃조건하보존1주。결론류식세포분석법검측NK세포화B세포，약표본불능급시처리의18℃보존병재96 h내검측；처리후적양본가재4℃조건하보존1주。
Objective To study the effect of storage temperature and time on the detection of NK cells and B-lymphocytes. Methods NK cells and B-lymphocytes in peripheral blood were detected by 6-color flow cytometry. The effect of storage temperature and time on the detection of NK cells and B-lymphocytes was observed. Results No change was found in the number of NK cells and B-lymphocytes in whole blood samples stored at 18℃. The number of NK cells and B-lymphocytes decreased after stored at low or high temperature. The whole blood samples that were fixed with paraformaldehyde and stained with fluorescent antibody could be stored for 7 days. Conclusion Flow cytometry can detect NK cells and B-lymphocytes. The samples can be stored at 18℃for 96 h when it is not able to treat them in time. The treated samples can be stored at 4℃for 7 days.