解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2014年
3期
261-265
,共5页
甄为%罗俊生%霍晓川%关宁%王之赫%邱建武
甄為%囉俊生%霍曉川%關寧%王之赫%邱建武
견위%라준생%곽효천%관저%왕지혁%구건무
羧酸酯酶%炎症%TLR-4%血管损伤
羧痠酯酶%炎癥%TLR-4%血管損傷
최산지매%염증%TLR-4%혈관손상
carboxylesterase%inflammation%TLR-4%vascular injury
目的：探讨羧酸酯酶1(carboxylesterase 1，CES1)对大鼠颈动脉内膜损伤后血管狭窄及炎症反应的影响。方法随机将大鼠分为3组，CES1组使用球囊导管制备颈总动脉损伤模型，在术后0 d、1 d、2 d尾经脉注射载有CES1的慢病毒载体促进CES1高表达；空载病毒组颈动脉损伤后注射空载病毒；空白对照组给予假手术处理。于术后第14天取材，HE染色后计算内膜增生情况，免疫组化和Western Blot检测TLR-4蛋白表达，实时荧光定量PCR测定TRL-4基因表达。结果HE染色表明CES1高表达能够明显抑制血管内膜损伤后的内膜增生，降低炎症反应，减少血管狭窄，免疫组化及实时荧光定量PCR和Western blot结果显示CES1高表达能够降低血管内膜中TLR-4的蛋白及基因表达。结论 CES1高表达能够下调损伤血管内TLR-4的表达从而抑制炎症反应，减轻血管损伤后狭窄。
目的：探討羧痠酯酶1(carboxylesterase 1，CES1)對大鼠頸動脈內膜損傷後血管狹窄及炎癥反應的影響。方法隨機將大鼠分為3組，CES1組使用毬囊導管製備頸總動脈損傷模型，在術後0 d、1 d、2 d尾經脈註射載有CES1的慢病毒載體促進CES1高錶達；空載病毒組頸動脈損傷後註射空載病毒；空白對照組給予假手術處理。于術後第14天取材，HE染色後計算內膜增生情況，免疫組化和Western Blot檢測TLR-4蛋白錶達，實時熒光定量PCR測定TRL-4基因錶達。結果HE染色錶明CES1高錶達能夠明顯抑製血管內膜損傷後的內膜增生，降低炎癥反應，減少血管狹窄，免疫組化及實時熒光定量PCR和Western blot結果顯示CES1高錶達能夠降低血管內膜中TLR-4的蛋白及基因錶達。結論 CES1高錶達能夠下調損傷血管內TLR-4的錶達從而抑製炎癥反應，減輕血管損傷後狹窄。
목적：탐토최산지매1(carboxylesterase 1，CES1)대대서경동맥내막손상후혈관협착급염증반응적영향。방법수궤장대서분위3조，CES1조사용구낭도관제비경총동맥손상모형，재술후0 d、1 d、2 d미경맥주사재유CES1적만병독재체촉진CES1고표체；공재병독조경동맥손상후주사공재병독；공백대조조급여가수술처리。우술후제14천취재，HE염색후계산내막증생정황，면역조화화Western Blot검측TLR-4단백표체，실시형광정량PCR측정TRL-4기인표체。결과HE염색표명CES1고표체능구명현억제혈관내막손상후적내막증생，강저염증반응，감소혈관협착，면역조화급실시형광정량PCR화Western blot결과현시CES1고표체능구강저혈관내막중TLR-4적단백급기인표체。결론 CES1고표체능구하조손상혈관내TLR-4적표체종이억제염증반응，감경혈관손상후협착。
Objective To study the effect of carboxylesterase 1 (CES1) on stenosis and inflammatory reactions in rats after carotid artery injury. Methods Rats were randomly divided into CES1 group, non-virus loading group and blank control group. Rats in CES1 group were injected with slow virus vectors containing CES1 via the tail vein on days 0, 1, and 2 after the common carotid artery injury model was established. Rats in non-virus loading group were injected with slow virus vectors containing no CES1 after carotid artery injury. Rats in control group underwent sham operation. Carotid artery tissue samples were taken on day 14 after carotid artery injury. Hyperplasia of carotid artery intima was assayed with H&E staining. Expression of TLR-4 was detected by Western blot and RT-PCR, respectively, with immunohistochemical staining. Results Over-expressed CES1 could significantly inhibit hyperplasia of carotid artery intima, improve inflammatory reactions and reduce stenosis. RT-PCR and Western blot showed that over-expressed CES1 could down-regulate the expression of TLR-4 in carotid artery intima. Conclusion Over-expressed CES1 can inhibit the inflammatory reactions and improve stenosis after carotid artery injury by down-regulating the expression of TLR-4.
