河北中医
河北中醫
하북중의
HEBEI JORNAL OF TRADITIONAL CHINESE MEDICINE
2014年
3期
428-431,432
,共5页
姜洋%杨惠民%占程燕%李璐
薑洋%楊惠民%佔程燕%李璐
강양%양혜민%점정연%리로
小鼠,基因敲除%认知障碍,心理学%小鼠,转基因
小鼠,基因敲除%認知障礙,心理學%小鼠,轉基因
소서,기인고제%인지장애,심이학%소서,전기인
Mice,Gene knockout%Cognitive disorders%Psychology%Transgenosis
目的:通过研究补肾祛瘀化痰法对载脂蛋白E(ApoE)基因敲除小鼠认知功能的影响,探讨其组方通脉益智胶囊对认知障碍的疗效。方法将32只ApoE基因敲除小鼠随机分为4组,即成年中药组、老年中药组、成年模型组及老年模型组,每组8只;将16只相同遗传背景C57BL/6J小鼠随机分为2组,即成年正常组及老年正常组,每组8只。成年组小鼠至24周起、老年组小鼠至32周起,中药组以通脉益智胶囊灌胃12周,模型组与正常组以同容积0.9%氯化钠注射液灌胃12周,均每日1次。24周及36周时,成年组小鼠予跳台及避暗实验;32周及44周时,老年组小鼠予跳台及避暗实验。结果在24周给药前,成年中药组、成年模型组与成年正常组小鼠的错误次数比较差异均无统计学意义( P>0.05)。记忆成绩显示,成年中药组、成年模型组与成年正常组小鼠的错误次数均较第1 d有所减少,但比较差异无统计学意义(P>0.05);成年中药组、成年模型组与成年正常组小鼠的潜伏时间比较差异无统计学意义( P>0.05)。表明在小鼠24周龄时未出现明显认知障碍。在给药12周后,成年中药组、成年正常组错误次数均较成年模型组降低( P<0.05,P<0.01),成年中药组、成年正常组错误次数比较差异无统计学意义(P>0.05)。记忆成绩显示,成年中药组、成年正常组错误次数均较成年模型组降低(P<0.05,P<0.01),潜伏时间均较成年模型组增加(P<0.05,P<0.01);成年中药组、成年正常组错误次数、潜伏时间比较差异均无统计学意义(P>0.05)。表明在小鼠36周龄后已出现认知障碍,补肾祛痰化瘀法对认知障碍有一定改善。在32周给药前,老年中药组、老年模型组小鼠的错误次数均较老年正常组多(P<0.05),老年中药组与老年模型组之间比较差异无统计学意义(P>0.05)。第2 d 的记忆成绩显示,老年中药组、老年模型组小鼠的错误次数均较老年正常组多( P<0.05),潜伏时间均较老年正常组减少(P<0.05),老年中药组小鼠的错误次数、潜伏时间与老年模型组比较差异均无统计学意义( P>0.05)。表明32周龄时ApoE基因敲除小鼠已出现认知障碍。在给药12周后(第44周),老年中药组、老年正常组的错误次数均老年模型组减少(P<0.05,P<0.01),老年中药组的错误次数与老年正常组比较差异无统计学意义(P>0.05)。记忆成绩显示,老年中药组、老年模型组、老年正常组小鼠的错误次数均较前1 d有所减少,但比较差异均无统计学意义(P>0.05);老年中药组、老年正常组的错误次数均较老年模型组减少(P<0.01),潜伏时间均增加(P<0.01);老年中药组的错误次数、潜伏时间与老年正常组比较差异均无统计学意义( P>0.05)。表明在44周龄时ApoE基因敲除小鼠已出现明显的认知障碍,而补肾祛瘀化痰法则对认知障碍有明显的改善作用。结论老年ApoE基因敲除小鼠存在认知功能损害;补肾祛瘀化痰法干预老年ApoE基因敲除小鼠后可改善行为学表现,进而改善认知功能。
目的:通過研究補腎祛瘀化痰法對載脂蛋白E(ApoE)基因敲除小鼠認知功能的影響,探討其組方通脈益智膠囊對認知障礙的療效。方法將32隻ApoE基因敲除小鼠隨機分為4組,即成年中藥組、老年中藥組、成年模型組及老年模型組,每組8隻;將16隻相同遺傳揹景C57BL/6J小鼠隨機分為2組,即成年正常組及老年正常組,每組8隻。成年組小鼠至24週起、老年組小鼠至32週起,中藥組以通脈益智膠囊灌胃12週,模型組與正常組以同容積0.9%氯化鈉註射液灌胃12週,均每日1次。24週及36週時,成年組小鼠予跳檯及避暗實驗;32週及44週時,老年組小鼠予跳檯及避暗實驗。結果在24週給藥前,成年中藥組、成年模型組與成年正常組小鼠的錯誤次數比較差異均無統計學意義( P>0.05)。記憶成績顯示,成年中藥組、成年模型組與成年正常組小鼠的錯誤次數均較第1 d有所減少,但比較差異無統計學意義(P>0.05);成年中藥組、成年模型組與成年正常組小鼠的潛伏時間比較差異無統計學意義( P>0.05)。錶明在小鼠24週齡時未齣現明顯認知障礙。在給藥12週後,成年中藥組、成年正常組錯誤次數均較成年模型組降低( P<0.05,P<0.01),成年中藥組、成年正常組錯誤次數比較差異無統計學意義(P>0.05)。記憶成績顯示,成年中藥組、成年正常組錯誤次數均較成年模型組降低(P<0.05,P<0.01),潛伏時間均較成年模型組增加(P<0.05,P<0.01);成年中藥組、成年正常組錯誤次數、潛伏時間比較差異均無統計學意義(P>0.05)。錶明在小鼠36週齡後已齣現認知障礙,補腎祛痰化瘀法對認知障礙有一定改善。在32週給藥前,老年中藥組、老年模型組小鼠的錯誤次數均較老年正常組多(P<0.05),老年中藥組與老年模型組之間比較差異無統計學意義(P>0.05)。第2 d 的記憶成績顯示,老年中藥組、老年模型組小鼠的錯誤次數均較老年正常組多( P<0.05),潛伏時間均較老年正常組減少(P<0.05),老年中藥組小鼠的錯誤次數、潛伏時間與老年模型組比較差異均無統計學意義( P>0.05)。錶明32週齡時ApoE基因敲除小鼠已齣現認知障礙。在給藥12週後(第44週),老年中藥組、老年正常組的錯誤次數均老年模型組減少(P<0.05,P<0.01),老年中藥組的錯誤次數與老年正常組比較差異無統計學意義(P>0.05)。記憶成績顯示,老年中藥組、老年模型組、老年正常組小鼠的錯誤次數均較前1 d有所減少,但比較差異均無統計學意義(P>0.05);老年中藥組、老年正常組的錯誤次數均較老年模型組減少(P<0.01),潛伏時間均增加(P<0.01);老年中藥組的錯誤次數、潛伏時間與老年正常組比較差異均無統計學意義( P>0.05)。錶明在44週齡時ApoE基因敲除小鼠已齣現明顯的認知障礙,而補腎祛瘀化痰法則對認知障礙有明顯的改善作用。結論老年ApoE基因敲除小鼠存在認知功能損害;補腎祛瘀化痰法榦預老年ApoE基因敲除小鼠後可改善行為學錶現,進而改善認知功能。
목적:통과연구보신거어화담법대재지단백E(ApoE)기인고제소서인지공능적영향,탐토기조방통맥익지효낭대인지장애적료효。방법장32지ApoE기인고제소서수궤분위4조,즉성년중약조、노년중약조、성년모형조급노년모형조,매조8지;장16지상동유전배경C57BL/6J소서수궤분위2조,즉성년정상조급노년정상조,매조8지。성년조소서지24주기、노년조소서지32주기,중약조이통맥익지효낭관위12주,모형조여정상조이동용적0.9%록화납주사액관위12주,균매일1차。24주급36주시,성년조소서여도태급피암실험;32주급44주시,노년조소서여도태급피암실험。결과재24주급약전,성년중약조、성년모형조여성년정상조소서적착오차수비교차이균무통계학의의( P>0.05)。기억성적현시,성년중약조、성년모형조여성년정상조소서적착오차수균교제1 d유소감소,단비교차이무통계학의의(P>0.05);성년중약조、성년모형조여성년정상조소서적잠복시간비교차이무통계학의의( P>0.05)。표명재소서24주령시미출현명현인지장애。재급약12주후,성년중약조、성년정상조착오차수균교성년모형조강저( P<0.05,P<0.01),성년중약조、성년정상조착오차수비교차이무통계학의의(P>0.05)。기억성적현시,성년중약조、성년정상조착오차수균교성년모형조강저(P<0.05,P<0.01),잠복시간균교성년모형조증가(P<0.05,P<0.01);성년중약조、성년정상조착오차수、잠복시간비교차이균무통계학의의(P>0.05)。표명재소서36주령후이출현인지장애,보신거담화어법대인지장애유일정개선。재32주급약전,노년중약조、노년모형조소서적착오차수균교노년정상조다(P<0.05),노년중약조여노년모형조지간비교차이무통계학의의(P>0.05)。제2 d 적기억성적현시,노년중약조、노년모형조소서적착오차수균교노년정상조다( P<0.05),잠복시간균교노년정상조감소(P<0.05),노년중약조소서적착오차수、잠복시간여노년모형조비교차이균무통계학의의( P>0.05)。표명32주령시ApoE기인고제소서이출현인지장애。재급약12주후(제44주),노년중약조、노년정상조적착오차수균노년모형조감소(P<0.05,P<0.01),노년중약조적착오차수여노년정상조비교차이무통계학의의(P>0.05)。기억성적현시,노년중약조、노년모형조、노년정상조소서적착오차수균교전1 d유소감소,단비교차이균무통계학의의(P>0.05);노년중약조、노년정상조적착오차수균교노년모형조감소(P<0.01),잠복시간균증가(P<0.01);노년중약조적착오차수、잠복시간여노년정상조비교차이균무통계학의의( P>0.05)。표명재44주령시ApoE기인고제소서이출현명현적인지장애,이보신거어화담법칙대인지장애유명현적개선작용。결론노년ApoE기인고제소서존재인지공능손해;보신거어화담법간예노년ApoE기인고제소서후가개선행위학표현,진이개선인지공능。
Objective To explore the effects of the prescription ( Tongmai Yizhi capsule ) for tonifying kid-ney and removing stasis and reducing phlegm on cognitive function of ApoE knockout mice .Methods 32 ApoE knockout mice were randomly divided into four groups ,the treatment group and model group of senior and the treat-ment group and model group of elder ,8 mice in each group .And 16 C57BL/6J mice were divided into two groups as the normal groups in senior and elder 8 mice in each group.Mice in treatment group of senior received Tongmai Yizhi capsule from 24 weeks of mice for 12 weeks.Mice in treatment group of elder received Tong-mai Yizhi capsule from 32 weeks of mice for 12 weeks.Mice in model group and the normal group received the same volume of 0.9%sodium chloride injection for 12 weeks.At 24 weeks and 36 weeks,jumping and to avoid dark experi-ment of mice in group of senior were finished .At 32 weeks and 44 weeks,jumping and to avoid dark experiment of mice in group of elder were finished .Results There was no difference between the treatment group ,model group and normal group in senior on the number of errors before treatment (P<0.05).After continuous infusion 12 weeks,there were differences between the treatment group , model group and normal group in senior on the number of errors in memory performance (P<0.05,P<0.01).The the number of errors in memory performance in the treatment group and normal group in senior were lower than that in model group in senior .Latent time was increased as compared with that in model group (P<0.05,P<0.01).There was no difference between treatment group and normal group on the the number of errors and latent time (P>0.05).The results showed that ApoE knockout mice at 36-weeks had cog-nitive impairment .The method of tonifying kidney and removing stasis and reducing phlegm can improve cognitive im -pairment .Before 32-weeks the number of errors in model group and treatment group in elder were more than that in normal group in elder (P<0.05).There was no difference between model group and treatment group in elder on the number of errors (P>0.05).After continuous infusion 12 weeks, The number of errors in memory performance in the treatment group and normal group in elder were lower than that in model group in senior (P<0.05).Latent time was increased as compared with that in model group (P<0.05,P<0.01).The results showed that ApoE knockout mice at 44-weeks had cognitive impairment .The method of tonifying kidney and removing stasis and reducing phlegm can improve cognitive impairment .Conclusion ApoE knockout mice have cognitive impairment when they get old . The method of tonifying kidney and removing stasis and reducing phlegm can improve neurobehavioral manifestation and cognitive impairment on ApoE knockout mice .