化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2014年
3期
418-421
,共4页
咖啡酸%DNA%循环伏安法%相互作用
咖啡痠%DNA%循環伏安法%相互作用
가배산%DNA%순배복안법%상호작용
caffeic acid%DNA%cyclic voltammetry%interaction
用电化学方法研究了咖啡酸与DNA的相互作用,考察了扫速对咖啡酸与DNA相互作用的影响。实验表明,DNA存在使咖啡酸氧化峰的电位正移,咖啡酸的氧化峰峰电流减小,咖啡酸在320 nm吸收光谱的吸收峰降低,呈减色效应,且出现两个等电吸收点,说明咖啡酸与DNA的相互作用以嵌插作用为主。双链DNA (dsDNA)与咖啡酸的结合能力大于单链DNA(ssDNA),结合比为1∶1,结合常数β为2．32×106。
用電化學方法研究瞭咖啡痠與DNA的相互作用,攷察瞭掃速對咖啡痠與DNA相互作用的影響。實驗錶明,DNA存在使咖啡痠氧化峰的電位正移,咖啡痠的氧化峰峰電流減小,咖啡痠在320 nm吸收光譜的吸收峰降低,呈減色效應,且齣現兩箇等電吸收點,說明咖啡痠與DNA的相互作用以嵌插作用為主。雙鏈DNA (dsDNA)與咖啡痠的結閤能力大于單鏈DNA(ssDNA),結閤比為1∶1,結閤常數β為2．32×106。
용전화학방법연구료가배산여DNA적상호작용,고찰료소속대가배산여DNA상호작용적영향。실험표명,DNA존재사가배산양화봉적전위정이,가배산적양화봉봉전류감소,가배산재320 nm흡수광보적흡수봉강저,정감색효응,차출현량개등전흡수점,설명가배산여DNA적상호작용이감삽작용위주。쌍련DNA (dsDNA)여가배산적결합능력대우단련DNA(ssDNA),결합비위1∶1,결합상수β위2．32×106。
The interaction between caffeic acid and DNA was studied by cyclic voltammetry and uv-vis spectrcmetry. The results in-dicated that with the addition of DNA,the oxidation peak currents of caffeic acid decreased and peak potentials also shifted positive-ly,and the interaction between caffeic acid and dsDNA was stronger than that between caffeic acid and ssDNA. The results showed that caffeic acid react with DNA mainly in a mode of intercalation,which was comfirm by uv-vis spectrcmetry. The combining ratio of caffeic acid to dsDNA was 1∶1,with the combining constants 2. 32×106.
