CAJ | 학술논문

采用循环伏安法在铂电极表面形成聚间苯二胺-壳聚糖复合膜；以戊二醛为交联剂将葡萄糖氧化酶共价固定在复合膜上形成葡萄糖电化学生物传感器；运用扫描电镜考察复合膜的形貌特征；考察了壳聚糖用量和电位循环圈数对膜特性的影响；采用电流-时间法考察了该传感器对葡萄糖的电化学响应特性。结果表明,该传感器对葡萄糖有较快的响应速度(3．5s),在0．02m mol·L-1~1．27mmol·L-1浓度范围内响应电流与葡萄糖浓度成正比,检测极限为0．01m mol·L-1,样品测定的加标回收率为97．1%~102．5%。该传感器具有较好的选择性,对葡萄糖的测定具有较高的准确度与精密度。
채용순배복안법재박전겁표면형성취간분이알-각취당복합막；이무이철위교련제장포도당양화매공개고정재복합막상형성포도당전화학생물전감기；운용소묘전경고찰복합막적형모특정；고찰료각취당용량화전위순배권수대막특성적영향；채용전류-시간법고찰료해전감기대포도당적전화학향응특성。결과표명,해전감기대포도당유교쾌적향응속도(3．5s),재0．02m mol·L-1~1．27mmol·L-1농도범위내향응전류여포도당농도성정비,검측겁한위0．01m mol·L-1,양품측정적가표회수솔위97．1%~102．5%。해전감기구유교호적선택성,대포도당적측정구유교고적준학도여정밀도。
The poly( m-phenylenediamine)-chitosan composite membrane was formed on the surface of the platinum electrode by cy-clic voltammetry, then glucose oxidase was covalently immobilized onto the composite membrane by using glutaraldehyde as crosslinking agent to form glucose electrochemical biosensor. The appearance feature of the film was observed by scanning electron microscope. The influence of the amounts of chitosan and the number of potential cycle on the features of the membrane was investi-gated. The amperometric response characteristics of the biosensor for glucose was investigated by current-time method. The results showed that the biosensor displayed rapid response to glucose(3. 5s),and the response current was linear with glucose concentration over a range from 0. 02m mol·L-1 ~1. 27mmol·L-1 with a detection limit of 0. 01m mol·L-1 . The standard adding recovery ratio of glucose was 97. 1% ~102. 5%. The sensor has a good selectivity,a higher accuracy and precision for the determination of glucose.