食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
4期
44-48
,共5页
黑花生衣%黄酮类化合物%超声波%抗氧化
黑花生衣%黃酮類化閤物%超聲波%抗氧化
흑화생의%황동류화합물%초성파%항양화
Black peanut skin%flavonoid compounds%ultrasonic%antioxidation
研究超声波辅助提取黑花生衣中黄酮类化合物的工艺条件,探讨乙醇浓度、料液比、提取时间、提取温度、pH对提取效果的影响。确定影响黄酮提取效果的最主要因素是料液比,并得出黑花生衣中黄酮类化合物在超声波频率为50 kHz条件下提取的最佳工艺条件为:提取溶剂为60%乙醇,提取时间45 min ,提取温度40℃,料液比1∶300(g/mL),pH2.5。在此基础上,通过测定黑花生衣提取液对活性自由基的清除作用,探讨其抗氧化活性。结果表明,黑花生衣中黄酮类化合物对羟基自由基(·OH)、和DPPH·自由基皆具有显著的清除作用,且其清除作用与浓度之间呈量效关系。
研究超聲波輔助提取黑花生衣中黃酮類化閤物的工藝條件,探討乙醇濃度、料液比、提取時間、提取溫度、pH對提取效果的影響。確定影響黃酮提取效果的最主要因素是料液比,併得齣黑花生衣中黃酮類化閤物在超聲波頻率為50 kHz條件下提取的最佳工藝條件為:提取溶劑為60%乙醇,提取時間45 min ,提取溫度40℃,料液比1∶300(g/mL),pH2.5。在此基礎上,通過測定黑花生衣提取液對活性自由基的清除作用,探討其抗氧化活性。結果錶明,黑花生衣中黃酮類化閤物對羥基自由基(·OH)、和DPPH·自由基皆具有顯著的清除作用,且其清除作用與濃度之間呈量效關繫。
연구초성파보조제취흑화생의중황동류화합물적공예조건,탐토을순농도、료액비、제취시간、제취온도、pH대제취효과적영향。학정영향황동제취효과적최주요인소시료액비,병득출흑화생의중황동류화합물재초성파빈솔위50 kHz조건하제취적최가공예조건위:제취용제위60%을순,제취시간45 min ,제취온도40℃,료액비1∶300(g/mL),pH2.5。재차기출상,통과측정흑화생의제취액대활성자유기적청제작용,탐토기항양화활성。결과표명,흑화생의중황동류화합물대간기자유기(·OH)、화DPPH·자유기개구유현저적청제작용,차기청제작용여농도지간정량효관계。
Ultrasound-assisted extraction of flavonoid compounds from black peanut skin was studied in this paper,and we discussed the effect of the solvent concentration,extraction time,extraction temperature,ratio of solid and liquid and pH.The results showed that the most important factor was the ratio of solid and liquid.The optimum conditions were determined as:ultrasonic (50 kHz),60%ethanol,extraction time 45 min,extraction temperature 40℃,the ratio of solid and liquid 1∶300(g/mL) and pH 2.5.This paper also studied on the scavenging activity of the flavonoid compounds on the free radicals.The result showed that the flavonoid compounds can remove hydroxyl radicals and DPPH·, and the antioxidative activities increased with the increasing of the concentration.