食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
4期
5-7
,共3页
王天山%邢彦彬%谭志勇%唐望昌%苏美多%叶飞%李雯雯%宋小平
王天山%邢彥彬%譚誌勇%唐望昌%囌美多%葉飛%李雯雯%宋小平
왕천산%형언빈%담지용%당망창%소미다%협비%리문문%송소평
天然抗氧化剂%没食子酸三甲醚%自由基%紫外分光光度法
天然抗氧化劑%沒食子痠三甲醚%自由基%紫外分光光度法
천연항양화제%몰식자산삼갑미%자유기%자외분광광도법
natural antioxidants%3,4,5-trimethoxy benzoic acid%free radicals%UV spectrophotometry
探讨含芳香环抗氧化剂不同取代基对其抗氧化活性的影响，选择研究了五种含有苯环的天然化合物（Diayangambin，鹅掌楸树脂醇A二甲醚，N-反式-阿魏酰基酪胺，香兰素和没食子酸三甲醚）对自由基的清除能力，以邻苯三酚的自氧化产生的超氧阴离子及Fenton反应产生的羟基自由基作为模拟自由基，以VC作为对照品，采用紫外分光光度法测量这五种化合物对这两种自由基的清除作用。实验结果显示，各种天然化合物对这两种自由基的清除率均随浓度的增大而增加，其中前四种物质对超氧自由基的清除活性基本与VC相当，而没食子酸三甲醚对羟基自由基清除活性则最强。没食子酸三甲醚对羟基自由基的半清除浓度EC50值只有1.24×10-6 mg/mL，而VC的EC50值则高达3.48×10-4 mg/mL。研究发现在含苯环的抗氧化剂中，在苯环4位的取代基是甲氧基或者是羟基时对抗氧化活性的作用基本相似，而如果在苯环1位的取代基是羧基时则能明显增强其抗氧化活性。
探討含芳香環抗氧化劑不同取代基對其抗氧化活性的影響，選擇研究瞭五種含有苯環的天然化閤物（Diayangambin，鵝掌楸樹脂醇A二甲醚，N-反式-阿魏酰基酪胺，香蘭素和沒食子痠三甲醚）對自由基的清除能力，以鄰苯三酚的自氧化產生的超氧陰離子及Fenton反應產生的羥基自由基作為模擬自由基，以VC作為對照品，採用紫外分光光度法測量這五種化閤物對這兩種自由基的清除作用。實驗結果顯示，各種天然化閤物對這兩種自由基的清除率均隨濃度的增大而增加，其中前四種物質對超氧自由基的清除活性基本與VC相噹，而沒食子痠三甲醚對羥基自由基清除活性則最彊。沒食子痠三甲醚對羥基自由基的半清除濃度EC50值隻有1.24×10-6 mg/mL，而VC的EC50值則高達3.48×10-4 mg/mL。研究髮現在含苯環的抗氧化劑中，在苯環4位的取代基是甲氧基或者是羥基時對抗氧化活性的作用基本相似，而如果在苯環1位的取代基是羧基時則能明顯增彊其抗氧化活性。
탐토함방향배항양화제불동취대기대기항양화활성적영향，선택연구료오충함유분배적천연화합물（Diayangambin，아장추수지순A이갑미，N-반식-아위선기락알，향란소화몰식자산삼갑미）대자유기적청제능력，이린분삼분적자양화산생적초양음리자급Fenton반응산생적간기자유기작위모의자유기，이VC작위대조품，채용자외분광광도법측량저오충화합물대저량충자유기적청제작용。실험결과현시，각충천연화합물대저량충자유기적청제솔균수농도적증대이증가，기중전사충물질대초양자유기적청제활성기본여VC상당，이몰식자산삼갑미대간기자유기청제활성칙최강。몰식자산삼갑미대간기자유기적반청제농도EC50치지유1.24×10-6 mg/mL，이VC적EC50치칙고체3.48×10-4 mg/mL。연구발현재함분배적항양화제중，재분배4위적취대기시갑양기혹자시간기시대항양화활성적작용기본상사，이여과재분배1위적취대기시최기시칙능명현증강기항양화활성。
On the purpose of investigating the impact factors of anti-oxidant activities by aromatic antioxidants with different substitutents, five natural aromatic compounds (Diayangambin, Epiyangambin, N-trans-feruloyltyramine, Vanillin and 3,4,5-trimethoxy benzoic acid) were selected to be natural antioxidants to scavenge free radicals. The anti-oxidant activities were evaluated by UV spectrophotometry, applying vitamin C as control, and the model free radicals were superoxide anion free radical from pyrogallol autooxidation under alkaline condition, and hydroxyl radical from Fenton reaction. The experimental results showed that all five natural compounds indicated activities to these two free radicals, the former four compounds showed activities to superoxide anion free radical, which were nearly to that of vitamin C;furthermore, 3,4,5-trimethoxy benzoic acid showed very strong activity to hydroxyl radical with the EC50 value of 1.24×10-6 mg/mL, which was stronger than that of vitamin C with the EC50 value of 3.48×10-4 mg/mL. By comparing the anti-oxidant activities with the relevant natural compounds, the contribution to anti-oxidant activity of methoxyl group substituting at para-position of phenyl group was similar to that of hydroxyl group. Indeed, a strong electron-attracting group (such as carboxyl group), located at the primary position of benzene ring, can strengthen the anti-oxidant activity obviously.
