CAJ | 학술논문

建立一种通过易于保存的固体试剂来快速测定食品中过氧化氢的分光光度法。以固体酸氨基磺酸代替硫酸提供酸性环境，以草酸钛钾代替二氧化钛提供钛离子，酸性条件下钛离子与过氧化氢反应形成稳定的橙色络合物，通过分光光度计比色测定过氧化氢的含量。优化后的测定条件为：波长为390 nm，氨基磺酸和草酸钛钾的用量分别为100 mg和180 mg，反应温度为25℃，反应时间10 min。该方法在过氧化氢含量为1.0 mg/L~5.0 mg/L时与吸光度呈线性关系，标准曲线的相关系数R2=0.9994，最低检出限为0.45 mg/L，测定相对标准偏差小于3%，平均加标回收率为91.6%~106%。
건립일충통과역우보존적고체시제래쾌속측정식품중과양화경적분광광도법。이고체산안기광산대체류산제공산성배경，이초산태갑대체이양화태제공태리자，산성조건하태리자여과양화경반응형성은정적등색락합물，통과분광광도계비색측정과양화경적함량。우화후적측정조건위：파장위390 nm，안기광산화초산태갑적용량분별위100 mg화180 mg，반응온도위25℃，반응시간10 min。해방법재과양화경함량위1.0 mg/L~5.0 mg/L시여흡광도정선성관계，표준곡선적상관계수R2=0.9994，최저검출한위0.45 mg/L，측정상대표준편차소우3%，평균가표회수솔위91.6%~106%。
A kind of fast determination method of hydrogen peroxide in food by spectrophotometric with solid reagents was established in this paper. In this method, solid acid (amino sulfonic acid) was used to substitute for sulphuric acid to provide acid reaction condition and titanium potassium oxalate was used to substitute for titanium dioxide to provide titanium ion. The titanium ion was reacted with hydrogen peroxide under acid condition to form an orange complex and the color was determined by spectrophometric method to estimate hydrogen peroxide content. The optimum test conditions for hydrogen peroxide were determination wavelength of 390 nm, solid acid addition quantity of 100 mg, titanium potassium oxalate addition quantity of 180 mg, reaction temperature of 25℃and reaction time of 10 min. In this method, the hydrogen peroxide content within 1.0 to 5.0 mg/L had linear relationship with absorbance, and the correlation coefficient of the standard curve (R2) was 0.999 4. The minimum detection limit of the provided method was 0.45 mg/L, RSD was below 3 %, and the average recovery rate of the sample was under 91.6%to 106%.