CAJ | 학술논문

建立了一种新的有机相脂肪酶转酯化活性测定方法。以正己烷为溶剂，脂肪酶催化棕榈酸对硝基苯酯和正丁醇的转酯化反应为模型反应，通过测定反应液中310 nm下吸光值的变化计算反应转化率。以气相色谱法对新建的紫外分光光度法进行验证，分别采用这两种方法测定了七种商品化脂肪酶的转酯化活性，两种方法所得实验结果基本一致。利用紫外分光光度检测法考察了Lipozyme TL IM脂肪酶催化转酯化的时间进程及合成活性与酶量的关系，并对Lipozyme TL IM催化转酯化的性质(最适溶剂、酰基受体特异性、醇耐受性、最优反应温度和热力学稳定性)进行了表征。
건립료일충신적유궤상지방매전지화활성측정방법。이정기완위용제，지방매최화종려산대초기분지화정정순적전지화반응위모형반응，통과측정반응액중310 nm하흡광치적변화계산반응전화솔。이기상색보법대신건적자외분광광도법진행험증，분별채용저량충방법측정료칠충상품화지방매적전지화활성，량충방법소득실험결과기본일치。이용자외분광광도검측법고찰료Lipozyme TL IM지방매최화전지화적시간진정급합성활성여매량적관계，병대Lipozyme TL IM최화전지화적성질(최괄용제、선기수체특이성、순내수성、최우반응온도화열역학은정성)진행료표정。
A novel ultraviolet (UV) spectrophotometric method for evaluating lipase transesterification activi-ty in organic solvents is described. The model reaction was lipase-catalyzed transesterification between p-nitrophenyl palmitate (pNPP) and n-butanol in n-hexane at 30 °C. The conversion of pNPP to p-nitrophenol was monitored by measuring UV absorbance changes at 310 nm. Seven commercial lipases were tested using this method, and the results were similar to those obtained using gas chromatography. The UV spectrophotometric method was used to investigate the kinetics of the Lipozyme TL IM-catalyzed reaction of pNPP, and the correlation between synthetic activity and biocatalyst concentration was determined. The method was also used to investigate the Lipozyme TL IM-catalyzed transesterification properties, namely solvent effect, acyl acceptor speci-ficity, alcohol tolerance, optimum reaction temperature, and thermostability.