催化学报
催化學報
최화학보
CHINESE JOURNAL OF CATALYSIS
2014年
4期
524-531
,共8页
王敏%俞兰%李方珍%谢家庆
王敏%俞蘭%李方珍%謝傢慶
왕민%유란%리방진%사가경
氮杂大环%铈(III)%配合物%DNA%切割
氮雜大環%鈰(III)%配閤物%DNA%切割
담잡대배%시(III)%배합물%DNA%절할
Aza-cycle%Cerium%Complex DNA%Cleavage
以一种1,4,7,10,13,16,19,22,25,28,31-十一氮杂2,5,8,11,14,17,20,23,26,29,32-十一酰基-环三十三烷的衍生物(CYC)为配体,在溶液中和Ce(Ⅲ, M)离子形成氮杂大环铈配合物(M-CYC)作为仿生催化剂,通过紫外光谱法、荧光光谱法和凝胶电泳法研究了M-CYC配合物与DNA的相互作用.结果表明, M-CYC与小牛胸腺DNA (CT DNA)以嵌插方式相互作用,在pH =8.16的缓冲体系中,其结合常数Kb =2.0×104 L/mol.凝胶电泳结果表明,当自由基捕捉剂存在时, M-CYC可将超螺旋pUC19 DNA切割为缺刻型DNA,其切割方式为水解切割.
以一種1,4,7,10,13,16,19,22,25,28,31-十一氮雜2,5,8,11,14,17,20,23,26,29,32-十一酰基-環三十三烷的衍生物(CYC)為配體,在溶液中和Ce(Ⅲ, M)離子形成氮雜大環鈰配閤物(M-CYC)作為倣生催化劑,通過紫外光譜法、熒光光譜法和凝膠電泳法研究瞭M-CYC配閤物與DNA的相互作用.結果錶明, M-CYC與小牛胸腺DNA (CT DNA)以嵌插方式相互作用,在pH =8.16的緩遲體繫中,其結閤常數Kb =2.0×104 L/mol.凝膠電泳結果錶明,噹自由基捕捉劑存在時, M-CYC可將超螺鏇pUC19 DNA切割為缺刻型DNA,其切割方式為水解切割.
이일충1,4,7,10,13,16,19,22,25,28,31-십일담잡2,5,8,11,14,17,20,23,26,29,32-십일선기-배삼십삼완적연생물(CYC)위배체,재용액중화Ce(Ⅲ, M)리자형성담잡대배시배합물(M-CYC)작위방생최화제,통과자외광보법、형광광보법화응효전영법연구료M-CYC배합물여DNA적상호작용.결과표명, M-CYC여소우흉선DNA (CT DNA)이감삽방식상호작용,재pH =8.16적완충체계중,기결합상수Kb =2.0×104 L/mol.응효전영결과표명,당자유기포착제존재시, M-CYC가장초라선pUC19 DNA절할위결각형DNA,기절할방식위수해절할.
A new biomimetic catalyst (M-CYC) is formed from the combination of CYC (a derivative of 1,4,7,10,13,16,19,22,25,28,31-undecaaza-cyclotritriacontane-2,5,8,11,14,17,20,23,26,29,32-undeca acyl) and Ce(III, M) in aqueous solution. The interaction between this complex and DNA was studied using electronic absorption spectroscopy, fluorescence spectroscopy, and gel electrophoresis. The results indicated that M-CYC and calf thymus DNA (pUC19 DNA) interacted via intercalation. The apparent binding constant of the complex with DNA was determined to be 2.0×104 L/mol at pH 8.16. Based on gel electrophoresis analysis, M-CYC was shown to nick the circular plasmid pUC19 DNA in the presence of a free radical scavenger. The nicking of DNA serves as a model of hydrolyzed cleavage.
