中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2014年
5期
46-49
,共4页
豚鼠银屑病模型%白疕合剂%T细胞活化
豚鼠銀屑病模型%白疕閤劑%T細胞活化
돈서은설병모형%백비합제%T세포활화
The guinea pig models of psoriasis%Baibi mixture%The activation of T cells
目的：探讨中药白疕合剂对豚鼠银屑病模型 T 细胞活化的调节作用。方法：将豚鼠随机分为空白对照组、阴性对照组、白疕合剂低、中、高剂量组、消银颗粒组、阿维A组，采用ELISA法检测白疕合剂治疗后豚鼠银屑病模型血清中IL-2、INF-γ、IL-4、IL-10的水平，免疫组织化学法检测皮肤淋巴细胞相关抗原（CLA）的表达。结果：表明白疕合剂高、中、低剂量组血清中IL-2及INF-γ水平降低，同时IL-4及IL-10在血清中的水平升高，白疕合剂能抑制豚鼠皮损中CLA的表达。结论：中药白疕合剂对豚鼠银屑病模型有较好的治疗作用，其作用机理可能是通过调节T细胞的活化作用来治疗银屑病。
目的：探討中藥白疕閤劑對豚鼠銀屑病模型 T 細胞活化的調節作用。方法：將豚鼠隨機分為空白對照組、陰性對照組、白疕閤劑低、中、高劑量組、消銀顆粒組、阿維A組，採用ELISA法檢測白疕閤劑治療後豚鼠銀屑病模型血清中IL-2、INF-γ、IL-4、IL-10的水平，免疫組織化學法檢測皮膚淋巴細胞相關抗原（CLA）的錶達。結果：錶明白疕閤劑高、中、低劑量組血清中IL-2及INF-γ水平降低，同時IL-4及IL-10在血清中的水平升高，白疕閤劑能抑製豚鼠皮損中CLA的錶達。結論：中藥白疕閤劑對豚鼠銀屑病模型有較好的治療作用，其作用機理可能是通過調節T細胞的活化作用來治療銀屑病。
목적：탐토중약백비합제대돈서은설병모형 T 세포활화적조절작용。방법：장돈서수궤분위공백대조조、음성대조조、백비합제저、중、고제량조、소은과립조、아유A조，채용ELISA법검측백비합제치료후돈서은설병모형혈청중IL-2、INF-γ、IL-4、IL-10적수평，면역조직화학법검측피부림파세포상관항원（CLA）적표체。결과：표명백비합제고、중、저제량조혈청중IL-2급INF-γ수평강저，동시IL-4급IL-10재혈청중적수평승고，백비합제능억제돈서피손중CLA적표체。결론：중약백비합제대돈서은설병모형유교호적치료작용，기작용궤리가능시통과조절T세포적활화작용래치료은설병。
Objective: To investigate the regulatory effect of Baibi mixture on T cell activation in psoriasis guinea pig model. Methods:The guinea pigs were randomly divided into blank control group, negative control group, Baibi mixture of low, middle and high dose group, Xiaoyin granules group, acitretin group, ELISA method was used to observe the levels of psoriasis guinea pig model IL-2, INF- γ, IL-4, IL-10 in the serum after treatment. The expression of the cutaneous lymphocyte-associated antigen (CLA) was detected by immunohistochemical method. Results:The low-level of IL-2, INF-γand the high level of IL-4, IL-10 in Baibi mixture of high, middle and low dose group, Baibi mixture could inhibit the expression of the cutaneous lymphocyte-associated antigen (CLA) in the lesions of guinea pigs. Conclusion: Baibi mixture has good therapeutic effect on psoriasis guinea pig model, and its mechanism might be to treat psoriasis by activation of regulatory T cells.
