CAJ | 학술논문

目的：通过对脓毒症大鼠血清中MMP-9浓度的影响并结合肺脏形态学及超微结构的变化，探讨血必净注射液对肺脏的保护作用及其作用机制。方法选用雄性S-D大鼠45只随机分为3组：空白组（ Sham组）、盐水组（ NS组）、血必净组（ X组），每组15只。检测血清MMP-9水平、肺湿/干重比（ W/D）、HE染色法光镜观察肺脏形态学改变，透射电镜观察肺脏血管内皮细胞的超微结构。结果与Sham组比较，NS组、X组大鼠肺湿/干重比均明显增大（ P ＜0．01）；与NS组比较，X组肺组织湿/干重比显著减小（ P ＜0．01）。 NS组、X组血清中MMP-9水平均显著高于Sham组（ P ＜0．01）；与NS组比较，X组血清中MMP-9水平下降（ P ＜0．01）。 Sham组大鼠肺组织结构及血管内皮细胞结构基本正常；NS组有明显炎性反应及血管内皮细胞结构破坏等；X组改变明显减轻但较Sham组重。结论血必净注射液可减少血清MMP-9水平，从而减轻肺血管内皮细胞损伤，改善肺血管通透性，对肺脏有一定的保护作用。
목적：통과대농독증대서혈청중MMP-9농도적영향병결합폐장형태학급초미결구적변화，탐토혈필정주사액대폐장적보호작용급기작용궤제。방법선용웅성S-D대서45지수궤분위3조：공백조（ Sham조）、염수조（ NS조）、혈필정조（ X조），매조15지。검측혈청MMP-9수평、폐습/간중비（ W/D）、HE염색법광경관찰폐장형태학개변，투사전경관찰폐장혈관내피세포적초미결구。결과여Sham조비교，NS조、X조대서폐습/간중비균명현증대（ P ＜0．01）；여NS조비교，X조폐조직습/간중비현저감소（ P ＜0．01）。 NS조、X조혈청중MMP-9수평균현저고우Sham조（ P ＜0．01）；여NS조비교，X조혈청중MMP-9수평하강（ P ＜0．01）。 Sham조대서폐조직결구급혈관내피세포결구기본정상；NS조유명현염성반응급혈관내피세포결구파배등；X조개변명현감경단교Sham조중。결론혈필정주사액가감소혈청MMP-9수평，종이감경폐혈관내피세포손상，개선폐혈관통투성，대폐장유일정적보호작용。
Objective To investigate the changes of serum levels of MMP-9 and the pathological changes of lung tissue in rats with sepsis induced by CLP ,and to explore the protection effect and action mechanism of Xuebijing injection . Methods Forty five male SD rats were randomly divided into 3 groups: blank group,NS group and Xuebijing group .Serum MMP-9 levels and the ratio of lung wet/dry weight were determined .The pathologic changes of lung were observed by means of light microscopy and transmission electron microscopy .Results As compared with that in blank group ,the ratio of lung wet/dry weight in NS group and Xuebijing group was significantly increased ( P <0.01),however,as compared with that in NS group,the ratio of lung wet/dry weight in Xuebijing group was significantly decreased ( P <0.01).The serum levels of MMP-9 in NS group and Xuebijing group were significantly higher than those in blank group ( P <0.01).As compared with those in NS group,the serum levels of MMP-9 in Xuebijing group was significantly decreased ( P <0.01).The lung tissue structures and vascular endothelial cell structures in blank group were basically normal ,however ,obvious inflammatory reaction and vascular endothelial cell structure injury were found in NS group , which in Xuebijing group were obviously relieved , however,which were severer than those in blank group .Conclusion Xuebijing injection can reduce MMP-9 levels, as a result,which can relieve lung vascular endothelial cell injury and improve pulmonary vasopermeability ,so Xuebijing injection has certain protection effect on lungs .