CAJ | 학술논문

目的：探讨胆囊收缩素-8（cholecystokinin-8，CCK-8）对高血压性大鼠脑出血后血肿周围组织核因子-κB （ Necular Factor Kappa B ，NF-κB）活性的影响。方法取成年SD大鼠72只，体重250～300 g，随机分假手术组（ n ＝12）、脑出血组（ n ＝24）和CCK-8治疗组（ n ＝24）。采用电泳迁移率改变分析方法（electrophoretic mobility shift assay ， EMSA）检测核内NF-κB的活性，用免疫组化方法观察NF-κB在细胞内的表达情况，并用干湿重法测量出血侧脑组织含水量。结果脑出血组各时间点NF-κB的活性以及脑含水量较假手术组和CCK-8治疗均有明显增高（ P ＜0妹．05）。结论脑出血后，脑组织NF-κB的表达明显增加，CCK-8可以有效的抑制NF-κB的活性，这对于减轻NF-κB在脑出血后介导的继发性炎性损害起到了积极的作用。
목적：탐토담낭수축소-8（cholecystokinin-8，CCK-8）대고혈압성대서뇌출혈후혈종주위조직핵인자-κB （ Necular Factor Kappa B ，NF-κB）활성적영향。방법취성년SD대서72지，체중250～300 g，수궤분가수술조（ n ＝12）、뇌출혈조（ n ＝24）화CCK-8치료조（ n ＝24）。채용전영천이솔개변분석방법（electrophoretic mobility shift assay ， EMSA）검측핵내NF-κB적활성，용면역조화방법관찰NF-κB재세포내적표체정황，병용간습중법측량출혈측뇌조직함수량。결과뇌출혈조각시간점NF-κB적활성이급뇌함수량교가수술조화CCK-8치료균유명현증고（ P ＜0매．05）。결론뇌출혈후，뇌조직NF-κB적표체명현증가，CCK-8가이유효적억제NF-κB적활성，저대우감경NF-κB재뇌출혈후개도적계발성염성손해기도료적겁적작용。
Objective To investigate the effect of cholecystokinin-8 ( CCK-8 ) on the changes of Necular Factor Kappa B ( NF-κB) activity in rats with hypertensive intracerebral hemorrhage .Methods Seventy-two adult Sprague-Dawley rats,weighted 250 ~300 g, were randomly divided into three groups: control group , cerebral hemorrhage group and CCK-8 treatment group.The activity of NF-κB was detected by electrophoretic mobility shift assay ( EMSA) and the expression of NF-κB in cells was detected by immunohistochemical methods ,and water content in brain tissue of injury part was measured . Results The activity of NF-κB and brain water content in cerebral hemorrhage group were significantly increased , as compared with those in control group and CCK-8 group ( P <0.05).Conclusion The expression levels of NF-κB are obviously increased immediately after intracerebral hemorrhage ,and CCK-8 can inhibit effectively the activity of NF-κB,which relieves the damage of secondary inflammatory reaction induced by NF-κB after intracerebral hemorrhage .