CAJ | 학술논문

目的探讨支气管肺泡灌洗液(BALF)支原体DNA检测对支原体肺炎的早期诊断价值.方法对103例肺炎患儿行BALF支原体DNA定量检测,同时对患儿的血清行支原体IgM定性检测,并以55例支气管异物患儿作为对照组.结果肺炎患儿BALF支原体DNA阳性率为32.43％,血清支原体IgM抗体阳性率为19.43％,两种支原体阳性率明显高于对照组(x2=13.00、4.11,均P＜0.05)；且BALF支原体DNA阳性率明显高于血清支原体IgM阳性率(x2=4.92,P＜0.05).结论 BALF支原体DNA定量检测较传统血清支原体IgM定性检测而言,标本的留取相应难度较大,但可避免血清IgM空窗期结果的假阴性,对MPP患儿有更高的诊断价值.
목적 탐토지기관폐포관세액(BALF)지원체DNA검측대지원체폐염적조기진단개치.방법 대103례폐염환인행BALF지원체DNA정량검측,동시대환인적혈청행지원체IgM정성검측,병이55례지기관이물환인작위대조조.결과 폐염환인BALF지원체DNA양성솔위32.43％,혈청지원체IgM항체양성솔위19.43％,량충지원체양성솔명현고우대조조(x2=13.00、4.11,균P＜0.05)；차BALF지원체DNA양성솔명현고우혈청지원체IgM양성솔(x2=4.92,P＜0.05).결론 BALF지원체DNA정량검측교전통혈청지원체IgM정성검측이언,표본적류취상응난도교대,단가피면혈청IgM공창기결과적가음성,대MPP환인유경고적진단개치.
Objective To study the value of MP-DNA in the early diagnosis of pneumonia by analyzing the result of MP-DNA in bronchoalveolar lavage fluid(BALF) and MP-IgM antibodies in serum.Methods 103 hospitalized children were detected for MP-DNA in BALF(positive:DNA copies ＞ 102/ml) and MP-IgM antibodies in serum.Results The MP-DNA positive in BALF and the MP-IgM positive in serum in children with pneumoina were significantly higher than those in children with foreign body in bronchus (x2 =13.00,4.11,all P ＜ 0.05).33.01％ of 103 children was MP-DNA positive,19.43％ of 103 children was MP-IgM positive.The MP-DNA positive in BALF was significantly higher than the MP-IgM positive in serum(x2 =4.92,P ＜0.05).Conclusion The samples of MP-DNA in BALF were collected more difficulty than the samples of MP-IgM in serum,but the detection of MP-DNA can avoid the false negative,which may be of value in the diagnosis and therapy of MPP.