中国保健营养（中旬刊）
中國保健營養（中旬刊）
중국보건영양（중순간）
China Hwalth Care & nutrition
2013年
11期
780-781
,共2页
溃疡性结肠炎%免疫紊乱%综合护理
潰瘍性結腸炎%免疫紊亂%綜閤護理
궤양성결장염%면역문란%종합호리
Ulcerative Colitis%T cellSubsets%Comprehensive Nursing
目的：分析溃疡性结肠炎患者外周血中T细胞亚群数量和比例的变化，观察综合护理模式对其疗效的作用。方法：选择20例UC患者，分离外周血单核细胞(PBMC），采用流式细胞术(FCM)检测Th1、Th2、Th17细胞的数量及Th1/Th2比值，ELISA法测检UC患者综合护护理前后PBMC培养上清中IFN-γ、IL-17、IL-4细胞因子水平，用采用综合护理模式观察其效果,与同期选择的健康对照组比较分析。结果：UC患者综合护理前PBMC中IFN-γ+Th1、IL-17+Th17细胞的数量，Th1/Th2比值均高于健康对照组，差别有统计学意义；综合护理处理后，Th1、Th17细胞的数量及Th1/Th2比值的与对照组比较，差别均无统计学意义。综合护理处理前IFN-γ、IL-17水平高于健康对照组，差异均有统计学意义；综合护理处理后,细胞因子的差异均无统计学意义。结论：UC患者外周血免疫紊乱在其发病机制中发挥重要作用，采用综合护理模式可改善其免疫紊乱状态，有利于患者恢复健康。
目的：分析潰瘍性結腸炎患者外週血中T細胞亞群數量和比例的變化，觀察綜閤護理模式對其療效的作用。方法：選擇20例UC患者，分離外週血單覈細胞(PBMC），採用流式細胞術(FCM)檢測Th1、Th2、Th17細胞的數量及Th1/Th2比值，ELISA法測檢UC患者綜閤護護理前後PBMC培養上清中IFN-γ、IL-17、IL-4細胞因子水平，用採用綜閤護理模式觀察其效果,與同期選擇的健康對照組比較分析。結果：UC患者綜閤護理前PBMC中IFN-γ+Th1、IL-17+Th17細胞的數量，Th1/Th2比值均高于健康對照組，差彆有統計學意義；綜閤護理處理後，Th1、Th17細胞的數量及Th1/Th2比值的與對照組比較，差彆均無統計學意義。綜閤護理處理前IFN-γ、IL-17水平高于健康對照組，差異均有統計學意義；綜閤護理處理後,細胞因子的差異均無統計學意義。結論：UC患者外週血免疫紊亂在其髮病機製中髮揮重要作用，採用綜閤護理模式可改善其免疫紊亂狀態，有利于患者恢複健康。
목적：분석궤양성결장염환자외주혈중T세포아군수량화비례적변화，관찰종합호리모식대기료효적작용。방법：선택20례UC환자，분리외주혈단핵세포(PBMC），채용류식세포술(FCM)검측Th1、Th2、Th17세포적수량급Th1/Th2비치，ELISA법측검UC환자종합호호리전후PBMC배양상청중IFN-γ、IL-17、IL-4세포인자수평，용채용종합호리모식관찰기효과,여동기선택적건강대조조비교분석。결과：UC환자종합호리전PBMC중IFN-γ+Th1、IL-17+Th17세포적수량，Th1/Th2비치균고우건강대조조，차별유통계학의의；종합호리처리후，Th1、Th17세포적수량급Th1/Th2비치적여대조조비교，차별균무통계학의의。종합호리처리전IFN-γ、IL-17수평고우건강대조조，차이균유통계학의의；종합호리처리후,세포인자적차이균무통계학의의。결론：UC환자외주혈면역문란재기발병궤제중발휘중요작용，채용종합호리모식가개선기면역문란상태，유리우환자회복건강。
Objective:To determine the peripheral blood Th17 celland Th1/Th2 subsets in patients with Ulcerative colitis(UC) and explore the role of comprehensive nursing. Methods:The CD4+IFN-γ+T (Th1)、CD4+IL-4+T(Th2) CD4+IL-17+T(Th17) subsets of the peripheral blood from 20 patients with UC before and after comprehensive nursing and 20 healthy controls were analyzed by flow cytometry with bi-color immunofluorescent staining. The levels of IFN-γ、IL-4 and IL-17 were detected by ELISA assays in UC patients before and after comprehensive nursing. Results:The numbers of peripheral blood Th17 and the Th1 subsets were significantly increased in patients with UC in comparison with that of healthy controls (P﹤0.05),but there were no difference about Th2 subsets,the rate of Th1/Th2 increased before comprehensive nursing. But after comprehensive nursing , there were of no significance. Conclusion:The predominance of Th17 in peripheral blood of UC and Th1-dominated immunity may play an important role in the pathogenesis of UC,The comprehensive nursing model can improve the immune state of disorder, which is beneficial to the patients health.
