CAJ | 학술논문

本文对质谱法（MS）、液相色谱质谱联用法（LC-MS）、毛细管电泳质谱联用（CE-MS）法、光谱分析法等在多肽检测中的最新进展进行了综述。质谱检测多肽最大的优势在于稳定性、重现性好，准确性高。采用色谱或电泳分离技术与质谱联用可解决相同氨基酸组成但序列不同的多肽的检测。光谱技术检测多肽，通常无需样品预处理分离，且光谱技术设计的多肽传感方式灵活多变，但光谱技术往往需要借助其他方法才可对多肽进行定性分析，因此该方法不具有普遍性。
본문대질보법（MS）、액상색보질보련용법（LC-MS）、모세관전영질보련용（CE-MS）법、광보분석법등재다태검측중적최신진전진행료종술。질보검측다태최대적우세재우은정성、중현성호，준학성고。채용색보혹전영분리기술여질보련용가해결상동안기산조성단서렬불동적다태적검측。광보기술검측다태，통상무수양품예처리분리，차광보기술설계적다태전감방식령활다변，단광보기술왕왕수요차조기타방법재가대다태진행정성분석，인차해방법불구유보편성。
In this paper, a review was made on study of mass spectrometry, liquid chromatography-mass spectrometry (LC-MS), capillary electrophoresis-mass spectrometry (CE-MS), spectroscopic method and other approaches in detection of polypeptide. Mass spectrometry approach is characterized by good stability, reproducibility and accuracy. LC-MS or CE-MS can detect polypeptide with the same amino acid composition but different sequence of amino acid. Spectrometry can detect Polypeptide with great flexibility to transduce the detecting signals without sample pretreatment and separation, but it cannot conduct a qualitative analysis of polypeptide, which limits its wide application.