CAJ | 학술논문

以大岩桐（Sinningia speciosa）花蕾RNA为模板，利用RACE技术克隆了大岩桐 GA I同源基因的全长cDNA ，命名为 SsGA I（在GenBank中登录号为：FJ594773）．序列分析表明，SsGA I的 ORF长度为1689 bp ，编码562个氨基酸，含有典型的植物DELLA结构域以及GRAS结构域．蛋白同源性分析表明，SsGA I与葡萄的VvGA I及陆地棉的GhGA I相似性分别为68％、64％．SsGA I基因在花萼中的相对表达量最高，在茎中表达沉默．将 SsGA I置于CaM V35S启动子控制下在烟草中异位表达，转基因烟草表现出植株矮化，花瓣数增加，花期延迟等新表型，表明 SsGA I参与了转基因烟草株高及花器官的发育．
이대암동（Sinningia speciosa）화뢰RNA위모판，이용RACE기술극륭료대암동 GA I동원기인적전장cDNA ，명명위 SsGA I（재GenBank중등록호위：FJ594773）．서렬분석표명，SsGA I적 ORF장도위1689 bp ，편마562개안기산，함유전형적식물DELLA결구역이급GRAS결구역．단백동원성분석표명，SsGA I여포도적VvGA I급륙지면적GhGA I상사성분별위68％、64％．SsGA I기인재화악중적상대표체량최고，재경중표체침묵．장 SsGA I치우CaM V35S계동자공제하재연초중이위표체，전기인연초표현출식주왜화，화판수증가，화기연지등신표형，표명 SsGA I삼여료전기인연초주고급화기관적발육．
Taking the floral buds RNA of Sinningia speciosa ,the full-length cDNA of GA I homologous gene is cloned by rapid-amplification of cDNA ends (RACE) technology ,and named SsGA I .The SsGA I has an open reading frame (ORF) of 1689 bp length encoding 562 amino acids as well as the typical plant DELLA domain and GRAS domain .The protein homologous analysis indicates that SsGAI is similar to VvGAI(Vitis vinifera) and GhGAI(Gossypium hirsutum) with the similarity of 68% and 64% .SsGA I gene expressed highly in the sepals ,but is silence in the stem .Placing SsGA I under the control of CaMV35S promoter and ectopic expressing in tobaccos ,the transgenic tobacco plants exhibit novel phenotypes with dwarf plant ,increased petal number and late flowering .These results indicate that SsGA I is involved in the plant height and floral organ development of transgenic tobaccos .