CAJ | 학술논문

目的：研究手足口病患儿感染初期外周血中Th17与调节性T细胞（ Treg细胞）及相关细胞因子的水平变化。方法采用流式细胞术检测75名手足口病患儿（分为重症病例组和普通病例组）和30例正常儿童外周血中（ CD3＋CD8-IL-17＋） Th17及（ CD4＋CD25＋Foxp3＋） Treg细胞的变化，采用ELISA方法检测血清中IL-6、IL-10、IL-17、IL-23、TGF-β1的水平。结果手足口病患儿各组与正常对照组相比，Treg细胞百分率、Treg/Th17比率及TGF-β1、IL-10水平均明显降低（ F＝5．580、6．205、0．000、0．014，P均＜0．05），重症病例组中Th17细胞百分率明显升高（F＝3．189，P＜0．05），IL-17有增高趋势，但差异不大，各组IL-23差异也无统计学意义（ P＞0．05）；重症病例组IL-6的含量与普通病例组、正常对照组相比明显升高（F＝7．318，P＜0．05）。结论手足口病患儿感染初期外周血Treg/Th17细胞及相关细胞因子的水平出现变化，通过减少Treg细胞，增加Th17细胞，扩大炎症反应，促进抗病毒作用。
목적：연구수족구병환인감염초기외주혈중Th17여조절성T세포（ Treg세포）급상관세포인자적수평변화。방법채용류식세포술검측75명수족구병환인（분위중증병례조화보통병례조）화30례정상인동외주혈중（ CD3＋CD8-IL-17＋） Th17급（ CD4＋CD25＋Foxp3＋） Treg세포적변화，채용ELISA방법검측혈청중IL-6、IL-10、IL-17、IL-23、TGF-β1적수평。결과수족구병환인각조여정상대조조상비，Treg세포백분솔、Treg/Th17비솔급TGF-β1、IL-10수평균명현강저（ F＝5．580、6．205、0．000、0．014，P균＜0．05），중증병례조중Th17세포백분솔명현승고（F＝3．189，P＜0．05），IL-17유증고추세，단차이불대，각조IL-23차이야무통계학의의（ P＞0．05）；중증병례조IL-6적함량여보통병례조、정상대조조상비명현승고（F＝7．318，P＜0．05）。결론수족구병환인감염초기외주혈Treg/Th17세포급상관세포인자적수평출현변화，통과감소Treg세포，증가Th17세포，확대염증반응，촉진항병독작용。
Objective To investigate the alterations of Treg cells , Th17 cells and related cyto-kines in peripheral blood of patients during the early stage of hand-foot-mouth disease ( HFMD) .Methods Flow cytometry was performed to analyze the percentages of Treg cells ( CD4+CD25+Foxp3 T cells) and Th17 cells ( CD3+CD8-IL-17+T cells) in peripheral blood samples collected from 49 patients with severe HFMD , 26 patients with common HFMD and 30 healthy children.The levels of IL-6, IL-10, IL-17, IL-23 and TGF-β1 in serum samples were measured by ELISA .Results The percentages of Treg cells , ratios of Treg/Th17 cells, serum levels of TGF-β1 and IL-10 in patients with HFMD were significantly decreased as compared with those of control group (F=5.580, 6.205, 0.000, 0.014, respectively, P<0.05).Patients with se-vere HFMD showed a significantly increased Th17 cells (F=3.189 P<0.05) and a tendency of enhanced IL-17 expression , but no significant differences with the levels of IL-17 were observed .No significant differ-ences with the expression of IL-23 in the patients among each group were detected (P>0.05).The levels of IL-6 in serum samples from severe disease group were obviously increased as compared with those of common HFMD group and control group (F=7.318, P<0.05).Conclusion The results of this study demonstrated that the levels of Treg , Th17 cells and some related cytokines were varied in peripheral blood of patients dur-ing the early stage of HFMD .Inflammatory responses were enhanced to promote anti-virus activities by sup-pressing Treg cells and stimulating Th17 cells.