中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
9期
680-686
,共7页
王欣茹%赵志强%王浩%刘佳%刘爱萍%于旭博%何彦林%乔瑞洁%林纪胜%谢贵林
王訢茹%趙誌彊%王浩%劉佳%劉愛萍%于旭博%何彥林%喬瑞潔%林紀勝%謝貴林
왕흔여%조지강%왕호%류가%류애평%우욱박%하언림%교서길%림기성%사귀림
ACYW135群脑膜炎球菌多糖疫苗%血清杀菌实验(SBA)%酶联免疫吸附试验
ACYW135群腦膜炎毬菌多糖疫苗%血清殺菌實驗(SBA)%酶聯免疫吸附試驗
ACYW135군뇌막염구균다당역묘%혈청살균실험(SBA)%매련면역흡부시험
Serogroup ACYW135 meningococcal polysaccharide vaccine%Serum bactericidal ac-tivity ( SBA)%Enzyme-linked immunoabsorbent assay ( ELISA)
目的：从血清抗体体外杀菌功能和IgG含量角度观察健康成人接种一剂ACYW135群脑膜炎球菌多糖疫苗后不同时间体内抗体的动态水平，探讨抗体功能和含量的相关性和疫苗的保护期限。方法对20名健康成人接种一剂四价脑膜炎多糖疫苗，分别采集免疫前和免疫后不同时期的血清。采用血清杀菌试验（ SBA）和标准定量酶联免疫吸附试验（ ELISA）检测血清中杀菌抗体滴度和抗荚膜多糖IgG抗体含量。结果成人免疫前血清中存在一定水平的抗A、C、Y和W135群荚膜多糖抗体，并且除C群外，血清中针对A、Y和W135群球菌的杀菌抗体滴度都较高。免疫15 d后，各群荚膜多糖抗体的含量和血清杀菌抗体滴度都较免疫前有了明显的升高（P＜0．05），并且在30～160 d维持在较高的水平；免疫后近3年时杀菌抗体滴度和IgG抗体浓度降至免疫前水平。血清杀菌抗体滴度和IgG抗体浓度之间相关关系较弱（r＜0．3，P＞0．05），但免疫前后不同时间的受试者的血清杀菌抗体几何均数滴度（GMTs）和IgG抗体几何均数浓度（GMCs）之间具有良好的相关关系（r＞0．7， P＜0．05）。结论受试者免疫前后不同时间血清杀菌抗体GMTs和IgG抗体GMCs，在评价疫苗效果时具有一致性。接种ACYW135群脑膜炎球菌多糖疫苗有效期大约3年。
目的：從血清抗體體外殺菌功能和IgG含量角度觀察健康成人接種一劑ACYW135群腦膜炎毬菌多糖疫苗後不同時間體內抗體的動態水平，探討抗體功能和含量的相關性和疫苗的保護期限。方法對20名健康成人接種一劑四價腦膜炎多糖疫苗，分彆採集免疫前和免疫後不同時期的血清。採用血清殺菌試驗（ SBA）和標準定量酶聯免疫吸附試驗（ ELISA）檢測血清中殺菌抗體滴度和抗莢膜多糖IgG抗體含量。結果成人免疫前血清中存在一定水平的抗A、C、Y和W135群莢膜多糖抗體，併且除C群外，血清中針對A、Y和W135群毬菌的殺菌抗體滴度都較高。免疫15 d後，各群莢膜多糖抗體的含量和血清殺菌抗體滴度都較免疫前有瞭明顯的升高（P＜0．05），併且在30～160 d維持在較高的水平；免疫後近3年時殺菌抗體滴度和IgG抗體濃度降至免疫前水平。血清殺菌抗體滴度和IgG抗體濃度之間相關關繫較弱（r＜0．3，P＞0．05），但免疫前後不同時間的受試者的血清殺菌抗體幾何均數滴度（GMTs）和IgG抗體幾何均數濃度（GMCs）之間具有良好的相關關繫（r＞0．7， P＜0．05）。結論受試者免疫前後不同時間血清殺菌抗體GMTs和IgG抗體GMCs，在評價疫苗效果時具有一緻性。接種ACYW135群腦膜炎毬菌多糖疫苗有效期大約3年。
목적：종혈청항체체외살균공능화IgG함량각도관찰건강성인접충일제ACYW135군뇌막염구균다당역묘후불동시간체내항체적동태수평，탐토항체공능화함량적상관성화역묘적보호기한。방법대20명건강성인접충일제사개뇌막염다당역묘，분별채집면역전화면역후불동시기적혈청。채용혈청살균시험（ SBA）화표준정량매련면역흡부시험（ ELISA）검측혈청중살균항체적도화항협막다당IgG항체함량。결과성인면역전혈청중존재일정수평적항A、C、Y화W135군협막다당항체，병차제C군외，혈청중침대A、Y화W135군구균적살균항체적도도교고。면역15 d후，각군협막다당항체적함량화혈청살균항체적도도교면역전유료명현적승고（P＜0．05），병차재30～160 d유지재교고적수평；면역후근3년시살균항체적도화IgG항체농도강지면역전수평。혈청살균항체적도화IgG항체농도지간상관관계교약（r＜0．3，P＞0．05），단면역전후불동시간적수시자적혈청살균항체궤하균수적도（GMTs）화IgG항체궤하균수농도（GMCs）지간구유량호적상관관계（r＞0．7， P＜0．05）。결론수시자면역전후불동시간혈청살균항체GMTs화IgG항체GMCs，재평개역묘효과시구유일치성。접충ACYW135군뇌막염구균다당역묘유효기대약3년。
Objective To dynamically analyze the antibodies with regard to in vitro serum bacteri-cidal activity and the quantity of IgG in healthy adults received one dose of immunization with ACYW 135 me-ningococcal polysaccharide vaccine .To investigate the term of protection with polysaccharide vaccine and the correlation between bactericidal titer and IgG concentration .Methods Twenty healthy adults were given one dose of immunization with quadrivalent meningococcal polysaccharide vaccine .Serum samples were col-lected before and after vaccination at specific time points .Test for serum bactericidal activity ( SBA ) and quantitative ELISA were performed to detect bactericidal titers and IgG concentrations in serum samples .Re-sults Certain levels of antibodies against capsular polysaccharides of serogroup A , C, Y and W135 strains had already existed prior to immunization .Moreover, bactericidal titers against serogroup A , Y and W135 strains except serogroup C strain were relatively high .Specific IgG concentrations and bactericidal titers for all serogroup stains were significantly increased on day 15 after vaccination (P<0.05), and then sustained at a high level during a time period from day 30 to day 160.Both IgG concentrations and bactericidal titers dropped to the levels similar to that before preimmunization in about 3 years.No significant correlation was observed between bactericidal titers and IgG concentrations (r<0.3, P>0.05).However, a close correla-tion was demonstrated between GMTs and GMCs of serum samples (r>0.7, P<0.05).Conclusion The geometric mean titers ( GMTs) and geometric mean concentrations ( GMCs) of serum samples collected be-fore and after vaccination at different time points were reliable and consistent parameters for the evaluation of vaccine .The term of protection of quadivalent meningococcal polysaccharide vaccine was about 3 years upon a single dose of immunization .
