国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
10期
1765-1769
,共5页
陈华新%邵伯棕%陈宣辰%周维明%张意
陳華新%邵伯棕%陳宣辰%週維明%張意
진화신%소백종%진선신%주유명%장의
B7-H1%树突状细胞%腺病毒%甲状腺相关性眼病%负向免疫调控
B7-H1%樹突狀細胞%腺病毒%甲狀腺相關性眼病%負嚮免疫調控
B7-H1%수돌상세포%선병독%갑상선상관성안병%부향면역조공
B7-H1%dendritic cells%adenovirus vector%thyroid-associated ophthalmopathy%negative immun-oregulation
目的：构建表达小鼠B7-H1基因的腺病毒载体,转染修饰树突状细胞,并研究该细胞对小鼠甲状腺相关性眼病( thyroid-associated ophthalmopathy,TAO)的治疗效应。方法：设计并构建小鼠B7-H1的腺病毒表达载体,转染小鼠骨髓来源的树突状细胞,检测该树突状细胞的表型和功能,鉴定其对免疫应答的负向调控能力,采用实验动物模型观察B7-H1基因修饰的树突状细胞在体内治疗TAO的效果。<br> 结果：成功构建出具有良好B7-H1表达效力的腺病毒载体,病毒滴度为1.8伊109 PFU/mL,转染腺病毒的小鼠骨髓来源的树突状细胞表现出调节性树突状细胞性能,能够负向抑制免疫应答；在动物模型中使用该型树突状细胞可以有效控制甲状腺眼病的发生发展。<br> 结论：成功构建了表达小鼠B7-H1基因的腺病毒表达载体,转染了该载体的树突状细胞具有调节性树突状细胞的性能,抑制正向免疫应答,能够有效抑制甲状腺眼病的发生发展,揭示基因修饰的树突状细胞可能成为治疗甲状腺眼病的潜在生物制剂。
目的：構建錶達小鼠B7-H1基因的腺病毒載體,轉染脩飾樹突狀細胞,併研究該細胞對小鼠甲狀腺相關性眼病( thyroid-associated ophthalmopathy,TAO)的治療效應。方法：設計併構建小鼠B7-H1的腺病毒錶達載體,轉染小鼠骨髓來源的樹突狀細胞,檢測該樹突狀細胞的錶型和功能,鑒定其對免疫應答的負嚮調控能力,採用實驗動物模型觀察B7-H1基因脩飾的樹突狀細胞在體內治療TAO的效果。<br> 結果：成功構建齣具有良好B7-H1錶達效力的腺病毒載體,病毒滴度為1.8伊109 PFU/mL,轉染腺病毒的小鼠骨髓來源的樹突狀細胞錶現齣調節性樹突狀細胞性能,能夠負嚮抑製免疫應答；在動物模型中使用該型樹突狀細胞可以有效控製甲狀腺眼病的髮生髮展。<br> 結論：成功構建瞭錶達小鼠B7-H1基因的腺病毒錶達載體,轉染瞭該載體的樹突狀細胞具有調節性樹突狀細胞的性能,抑製正嚮免疫應答,能夠有效抑製甲狀腺眼病的髮生髮展,揭示基因脩飾的樹突狀細胞可能成為治療甲狀腺眼病的潛在生物製劑。
목적：구건표체소서B7-H1기인적선병독재체,전염수식수돌상세포,병연구해세포대소서갑상선상관성안병( thyroid-associated ophthalmopathy,TAO)적치료효응。방법：설계병구건소서B7-H1적선병독표체재체,전염소서골수래원적수돌상세포,검측해수돌상세포적표형화공능,감정기대면역응답적부향조공능력,채용실험동물모형관찰B7-H1기인수식적수돌상세포재체내치료TAO적효과。<br> 결과：성공구건출구유량호B7-H1표체효력적선병독재체,병독적도위1.8이109 PFU/mL,전염선병독적소서골수래원적수돌상세포표현출조절성수돌상세포성능,능구부향억제면역응답；재동물모형중사용해형수돌상세포가이유효공제갑상선안병적발생발전。<br> 결론：성공구건료표체소서B7-H1기인적선병독표체재체,전염료해재체적수돌상세포구유조절성수돌상세포적성능,억제정향면역응답,능구유효억제갑상선안병적발생발전,게시기인수식적수돌상세포가능성위치료갑상선안병적잠재생물제제。
AIM:To construct adenovirus vector expressing mice B7-H1 gene, transfect dendritic cells ( DCs ) , and to study the therapeutic effect of modified DC on thyroid-associated ophthalmopathy ( TAO) in mice. <br> METHODS: We designed and constructed B7-H1 gene adenovirus expression vector, and transfected DCs from mouse bone marrow, tested the phenotype and function of modified DCs, identificated its negative regulation to immune responses. The modified DCs were infected the sicked mice. And then the immunotherapeutic effect of modified DCs to TAO were tested. <br> RESULTS: B7 - H1 gene adenovirus vector was constructed and transfected DCs from bone marrow. The titer of the recombinant adenovirus was 1. 8í109 PFU/mL. B7-H1 gene modified DCs characteristics of regulatory DCs, could inhibit positive immune responses. The inhibition proceeding of TAO into mice infected modified DCs, was obviously prior to the control mice. The gene modified DCs, maybe become the new immunotherapy biological agent to thy TAO. <br> CONCLUSION: We constructed the expression of mouse B7 - H1 gene adenovirus expressed vector successfully, transfected DCs, by vector have properties of regulatory DCs, inhibiting positive immune response and the occurrence and development of thyroid eye disease. Gene modified DCs, reveal potent to the treatment of thyroid eye disease.
