CAJ | 학술논문

目的研究Nitrofen诱导先天性膈疝(CDH)模型与正常大鼠胎肺中微小RNA(miRNA)的差异表达,寻找与大鼠CDH形成相关的miRNAs并用软件预测其靶基因.方法利用miRNA芯片检测Nitrofen诱导CDH大鼠模型及正常对照组大鼠胎肺中miRNAs的表达并对其表达谱进行差异性分析;挑选芯片结果中CDH组显著下调的miR-33并采用qRT-PCR进行验证;运用生物信息学软件预测可能调控的靶基因.结果通过对CDH和正常大鼠胎肺中miRNA表达谱的差异分析,CDH组中8个上调如miR-3588、miR-382*等,9个下调如miR-33、miR-193等.qRT-PCR检测显示,miR-33在CDH组中约为正常组的0.64倍(P＜0.05),并对其进行靶基因预测,分别筛选出10个靶基因.结论 miR 33在CDH组中表达显著下调,可能通过调控其靶基因而参与实验动物CDH肺发育不良的发病机制.
목적 연구Nitrofen유도선천성격산(CDH)모형여정상대서태폐중미소RNA(miRNA)적차이표체,심조여대서CDH형성상관적miRNAs병용연건예측기파기인.방법 이용miRNA심편검측Nitrofen유도CDH대서모형급정상대조조대서태폐중miRNAs적표체병대기표체보진행차이성분석;도선심편결과중CDH조현저하조적miR-33병채용qRT-PCR진행험증;운용생물신식학연건예측가능조공적파기인.결과 통과대CDH화정상대서태폐중miRNA표체보적차이분석,CDH조중8개상조여miR-3588、miR-382*등,9개하조여miR-33、miR-193등.qRT-PCR검측현시,miR-33재CDH조중약위정상조적0.64배(P＜0.05),병대기진행파기인예측,분별사선출10개파기인.결론 miR 33재CDH조중표체현저하조,가능통과조공기파기인이삼여실험동물CDH폐발육불량적발병궤제.
Objective To study the differences in expression of microRNAs (miRNAs) between nitrofen induced CDH and normal rat,and to predict the abnormal expression of miRNA target genes.Methods miRNAs expression of 4 normal lung tissues,4 CDH lung tissues were detected with miRNA microarray chips and the expression level of miR-33 was confirmed by real-time PCR.Potential miRNA targets were analyzed by bioinformatics.Results The results showed that 8 miRNAs were significantly up-regulated (such as miR-3588,miR-382 *,etc.),and 9 miRNAs down regulated (such as miR-33,miR-193,etc).The expression of miR 33 was significantly reduced by 36％ in CDH rats when compared to normal tissue (P＜0.05).Ten potential target genes of miR-33 were predicted by informatics analysis.Conclusions miR 33 may be involved in the pathogenesis of pulmonary hypoplasia of CDH rat by regulating its target genes.