中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
10期
1374-1376
,共3页
抗原,CD%RNA,小分子干扰%上皮细胞/细胞学%生物转化%卵巢肿瘤/病理学
抗原,CD%RNA,小分子榦擾%上皮細胞/細胞學%生物轉化%卵巢腫瘤/病理學
항원,CD%RNA,소분자간우%상피세포/세포학%생물전화%란소종류/병이학
Antigens,CD%RNA,small interfering%Epithelial cells/cytology%Biotransformation%Ovarian neoplasms/ pathology
目的 探讨CD146-siRNA对卵巢癌细胞A2780上皮-间质转化的影响.方法 采用western blot和RT-PCR方法,检测低表达CD146对卵巢癌细胞E-cadherin、vimentin和fibronectin mRNA和蛋白表达水平的影响.结果 相对于空白对照组和RNA干扰阴性对照组,CD146-siRNA组E-cadherin mRNA和蛋白表达上调,而vimentin和fibronectin mRNA和蛋白表达下调(P＜0.05).结论 CD146-siRNA抑制卵巢癌细胞A2780的上皮-间质转化.
目的 探討CD146-siRNA對卵巢癌細胞A2780上皮-間質轉化的影響.方法 採用western blot和RT-PCR方法,檢測低錶達CD146對卵巢癌細胞E-cadherin、vimentin和fibronectin mRNA和蛋白錶達水平的影響.結果 相對于空白對照組和RNA榦擾陰性對照組,CD146-siRNA組E-cadherin mRNA和蛋白錶達上調,而vimentin和fibronectin mRNA和蛋白錶達下調(P＜0.05).結論 CD146-siRNA抑製卵巢癌細胞A2780的上皮-間質轉化.
목적 탐토CD146-siRNA대란소암세포A2780상피-간질전화적영향.방법 채용western blot화RT-PCR방법,검측저표체CD146대란소암세포E-cadherin、vimentin화fibronectin mRNA화단백표체수평적영향.결과 상대우공백대조조화RNA간우음성대조조,CD146-siRNA조E-cadherin mRNA화단백표체상조,이vimentin화fibronectin mRNA화단백표체하조(P＜0.05).결론 CD146-siRNA억제란소암세포A2780적상피-간질전화.
Objective To investigate the influence of CD146-siRNA on epithelial-mesenchymal transition in human ovarian cancer cell line A2780.Methods The influence of loss of CD146 on the mRNA and protein expressions of E-cadherin,vimentin,and fibronectin was evaluated by real-time polymerase chain reaction (RT-PCR) and Western blotting.Results Compared to non-transfected (control group) and those transfected with RNAi-NC (RNAi negative control group),the mRNA and protein expressions of Ecadherin were significantly increased in A2780 cell lines transfected with RNAi-CD146.The loss of CD146 expression down-regulated the mRNA and protein expression of vimentin and fibronectin(P ＜ 0.05).Conclusions CD146-siRNA can suppress epithelial-mesenchymal transition in human ovarian cancer cell line A2780.
