粮油加工（电子版）
糧油加工（電子版）
량유가공（전자판）
Cereals and Oils Processing（Electronic Version）
2014年
3期
53-57
,共5页
孙立斌%刘飞%解桂东%侯俊超%于殿宇
孫立斌%劉飛%解桂東%侯俊超%于殿宇
손립빈%류비%해계동%후준초%우전우
米曲霉%高温豆粕%发酵工艺%水解度
米麯黴%高溫豆粕%髮酵工藝%水解度
미곡매%고온두박%발효공예%수해도
Aspergillus oryzae%High-temperature soybean meal%Fermentation process%Hydrolysis degree
以高温豆粕为原料，以酶活力为指标，通过紫外诱变和遗传稳定性试验选育出遗传性能稳定的蛋白酶高产的米曲霉诱变菌株；以水解度为指标，优化发酵工艺条件，进一步提高高温豆粕的水解度。紫外诱变条件为20W紫外灯35cm处辐照180s，得出最佳的发酵培养基组成为葡萄糖3%和高温豆粕12%；采用单因素试验方法确定的培养条件为：发酵温度30℃、发酵时间48h、接种量9%、摇床速度160r/min 和起始 pH 值5.5，此条件下高温豆粕的水解度可达到24.05%，比优化前提高了3.86%。
以高溫豆粕為原料，以酶活力為指標，通過紫外誘變和遺傳穩定性試驗選育齣遺傳性能穩定的蛋白酶高產的米麯黴誘變菌株；以水解度為指標，優化髮酵工藝條件，進一步提高高溫豆粕的水解度。紫外誘變條件為20W紫外燈35cm處輻照180s，得齣最佳的髮酵培養基組成為葡萄糖3%和高溫豆粕12%；採用單因素試驗方法確定的培養條件為：髮酵溫度30℃、髮酵時間48h、接種量9%、搖床速度160r/min 和起始 pH 值5.5，此條件下高溫豆粕的水解度可達到24.05%，比優化前提高瞭3.86%。
이고온두박위원료，이매활력위지표，통과자외유변화유전은정성시험선육출유전성능은정적단백매고산적미곡매유변균주；이수해도위지표，우화발효공예조건，진일보제고고온두박적수해도。자외유변조건위20W자외등35cm처복조180s，득출최가적발효배양기조성위포도당3%화고온두박12%；채용단인소시험방법학정적배양조건위：발효온도30℃、발효시간48h、접충량9%、요상속도160r/min 화기시 pH 치5.5，차조건하고온두박적수해도가체도24.05%，비우화전제고료3.86%。
Using enzyme activity as index, Aspergillus oryzae strain with high level of producing proteinase was screened by UV mutagenesis and genetic stability test. Based on hydrolysis degree as surveil-lance index, medium com-positions and fermentation conditions were optimized to improve hydrolysis degree of high-temperature soybean meal. Conditions of UV mutagenesis were as follows: UV was 20W, irradiant distance was 35cm, irradiant time was 180s. Op-timal medium compositions were 3%sugar, 12%high denatured soy flakes. Cultural condition determined by single factor experiment was as follows: temperature30℃for 48 h, inoculum 9%, culture-age 36h, agitation 160r/min, and initial pH5.5. With described optimum medium and condition, hydrolysis degree of high-temperature soybean meal could reach 24.05%which was 3.86%higher than before.
