CAJ | 학술논문

目的：探讨MicroRNA-126（ miR-126）在人结肠癌细胞中的表达以及对结肠癌细胞生物学行为的影响。方法应用荧光PCR定量检测60例结肠癌患者的结肠癌组织以及癌旁组织中miR-126的表达，共60对，再测定5株结肠癌细胞（ SW480、HT29、HCT116、LOVO、SW620）中的表达情况；CCK-8法、划痕实验和Transwell实验检测细胞的增殖、迁移和侵袭能力，并进一步通过体外实验研究miR-126对结肠癌细胞生物学行为的影响。结果与癌旁组织细胞比较，miR-126在结肠癌细胞中表达下降（95．0％vs．55．0％， P ＜0．05），发生转移者与未发生转移者比较，差异有统计学意义（25．0％vs．75．0％， P ＜0．05）。 miR-126可以抑制SW480细胞的生长及其侵袭转移，有基质胶Tran-swell实验中，转染组及空白对照组细胞迁移数分别为（10．0±4．3）个和（258．0±30．6）个；无基质胶的Transwell实验中，分别为（84．0±13．2）个和（335．0±27．3）个，差异均有统计学意义（ P ＜0．05）。 miR-126高表达可以增强癌细胞对奥沙利铂的敏感性。结论 miR-126可明显抑制结肠癌细胞的发生发展，影响多种结肠癌细胞株生物学行为。
목적：탐토MicroRNA-126（ miR-126）재인결장암세포중적표체이급대결장암세포생물학행위적영향。방법응용형광PCR정량검측60례결장암환자적결장암조직이급암방조직중miR-126적표체，공60대，재측정5주결장암세포（ SW480、HT29、HCT116、LOVO、SW620）중적표체정황；CCK-8법、화흔실험화Transwell실험검측세포적증식、천이화침습능력，병진일보통과체외실험연구miR-126대결장암세포생물학행위적영향。결과여암방조직세포비교，miR-126재결장암세포중표체하강（95．0％vs．55．0％， P ＜0．05），발생전이자여미발생전이자비교，차이유통계학의의（25．0％vs．75．0％， P ＜0．05）。 miR-126가이억제SW480세포적생장급기침습전이，유기질효Tran-swell실험중，전염조급공백대조조세포천이수분별위（10．0±4．3）개화（258．0±30．6）개；무기질효적Transwell실험중，분별위（84．0±13．2）개화（335．0±27．3）개，차이균유통계학의의（ P ＜0．05）。 miR-126고표체가이증강암세포대오사리박적민감성。결론 miR-126가명현억제결장암세포적발생발전，영향다충결장암세포주생물학행위。
Objective To investigate the expression of MicroRNA -126 (miR-126) in human colon cancer cells and its effect on biological behavior of colon cancer cells .Methods Sixty patients with colon cancer were enrolled in the study . Fluorescence PCR was used to detect the expression of miR-126 in the cancerous tissues and in the tissues adjacent to carcino-ma.The expression of miR-126 in the cancerous tissues paired up with the expression of miR-126 in the tissues adjacent to carcinoma, and there were 60 pairs in total.The expression of miR-126 in 5 colon cancer cell lines ( SW480, HT29, HCT116, LOVO, SW620) was also detected.The proliferation, migration and invasion ability of the cells were measured u-sing CCK 8 assay, scratch experiments and Transwell assay , respectively.The effect of miR-126 on the biological behaviors of human colon cancer cells was further studied through the experiments in vitro .Results Compared with adjacent normal tissue cells, miR-126 was decreased (95.0%vs.55.0%, P <0.05) in colon cancer cells , and were obviously when cancer metas-tasis or invasion is particularly evident ,when compared with those without metasis ,there were significant difference (25.0%vs.75.0%, P <0.05).Studies have shown that miR-126 can inhibit the growth of SW480 cells and their invasion and metas-tasis, Transwell assay with matrigel showed the migration cell number in transfection group and blank control group was (10.0 ±4.3) and (258.0 ±30.6),while Transwell assay without matrigel showed the migration cell number was (84.0 ± 13.2) and (335.0 ±27.3) respectively,there were significant differences( P <0.05).miR-126 expression can enhance the sensitivity of cancer cells to oxaliplatin .Conclusion miR-126 can inhibit the development of colon cancer cells , the impact of a variety of strains of the biological behavior of colon cancer cells .