CAJ | 학술논문

目的：研究金属螯合剂氯碘羟喹（CQ）干预大鼠脑出血（ICH）后铁超载状态下铜蓝蛋白（Cp）的表达。方法将48只Wistar大鼠随机分为2组：对照组、干预组各24只，均采用立体定向技术向大鼠尾状核区注射胶原酶制备ICH模型，制模成功后干预组给予氯碘羟喹50 mg/kg灌胃，1次/12 h；对照组用等量生理盐水灌胃。2组在术后1、3、7、14 d不同时间点各取6只大鼠断头取脑，采用免疫组化染色和实时荧光定量PCR检测Cp的表达。结果ICH模型制备后，随着时间延长2组Cp阳性表达数显著增加，在第7天达峰值；与对照组相比，干预组大鼠脑组织内Cp在第1天无统计学差异（ P ＞0 D．05），在3～14 d的表达均升高（ P ＜0．05）。2组Cp mRNA表达水平高峰出现在第7天，且第3、7、14 d均高于第1天表达水平（ P ＜0．05）。与对照组比较，干预组第3、7、14天Cp mRNA表达水平较高（ P ＜0．05）。结论 CQ通过调节脑出血后脑组织内铜蓝蛋白的表达，可能加速脑组织内铁离子的清除从而发挥脑保护作用。
목적：연구금속오합제록전간규（CQ）간예대서뇌출혈（ICH）후철초재상태하동람단백（Cp）적표체。방법장48지Wistar대서수궤분위2조：대조조、간예조각24지，균채용입체정향기술향대서미상핵구주사효원매제비ICH모형，제모성공후간예조급여록전간규50 mg/kg관위，1차/12 h；대조조용등량생리염수관위。2조재술후1、3、7、14 d불동시간점각취6지대서단두취뇌，채용면역조화염색화실시형광정량PCR검측Cp적표체。결과ICH모형제비후，수착시간연장2조Cp양성표체수현저증가，재제7천체봉치；여대조조상비，간예조대서뇌조직내Cp재제1천무통계학차이（ P ＞0 D．05），재3～14 d적표체균승고（ P ＜0．05）。2조Cp mRNA표체수평고봉출현재제7천，차제3、7、14 d균고우제1천표체수평（ P ＜0．05）。여대조조비교，간예조제3、7、14천Cp mRNA표체수평교고（ P ＜0．05）。결론 CQ통과조절뇌출혈후뇌조직내동람단백적표체，가능가속뇌조직내철리자적청제종이발휘뇌보호작용。
Objective To study the effect of clioquinol on the expression of ceruloplasmin in experimental cerebral hemorrhage ( ICH) rats.Methods 48 Wistar rats were randomly divided into 2 groups: control group and the intervention group with each group of 24 rats, using stereotactic injection of collagenase preparation ICH model to the caudate nucleus of rats, after molding success the intervention group were given clioquinol 50 mg /kg orally every 12 h;intragastric saline control group.From each of 2 groups, six rats decapitated at different time points after 1, 3,7,14 d, using immunohistochemical stai-ning and real-time PCR detection of Cp .Results ICH model after preparation , two groups with time Cp positive expression was significantly increased in the first seven days of the peak;compared with the control group , the intervention group within the brain tissue of rats in the first Cp day showed no significant difference ( P >0.05), the expression at 3-14 d were higher ( P <0.05).2 groups'Cp mRNA expression levels peaked at 7 days, and the first 3,7,14 d were higher than the first day expression level ( P <0.05).Compared with the control group , the intervention group 3,7,14 day Cp mRNA expression lev-els were higher ( P <0.05).Conclusion CQ adjust cerebral hemorrhage by regulating the expression of ceruloplasmin in brain tissue, brain tissue may accelerate the removal of iron ions and thus play a protective role in the brain .