中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2014年
5期
28-29
,共2页
男科清热胶囊%黄芪甲苷%HPLC
男科清熱膠囊%黃芪甲苷%HPLC
남과청열효낭%황기갑감%HPLC
Nanke Qingre Capsules%Astragaloside IV%HPLC
目的：建立测定男科清热胶囊中黄芪甲苷含量的标准方法。方法高效液相色谱(HPLC)法；色谱柱为C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(35：65),检测波长为205 nm,柱温35℃,进样量10μl。结果黄芪甲苷进样量在2.51~12.55μg与峰面积有良好的线性关系(r=0.9990),平均回收率为97.48%(n=6)。结论该方法专属性强、重现性好、简便易行,可用于该品种的质量控制的重要组成部分。
目的：建立測定男科清熱膠囊中黃芪甲苷含量的標準方法。方法高效液相色譜(HPLC)法；色譜柱為C18柱(250 mm×4.6 mm,5μm),流動相為乙腈-水(35：65),檢測波長為205 nm,柱溫35℃,進樣量10μl。結果黃芪甲苷進樣量在2.51~12.55μg與峰麵積有良好的線性關繫(r=0.9990),平均迴收率為97.48%(n=6)。結論該方法專屬性彊、重現性好、簡便易行,可用于該品種的質量控製的重要組成部分。
목적：건립측정남과청열효낭중황기갑감함량적표준방법。방법고효액상색보(HPLC)법；색보주위C18주(250 mm×4.6 mm,5μm),류동상위을정-수(35：65),검측파장위205 nm,주온35℃,진양량10μl。결과황기갑감진양량재2.51~12.55μg여봉면적유량호적선성관계(r=0.9990),평균회수솔위97.48%(n=6)。결론해방법전속성강、중현성호、간편역행,가용우해품충적질량공제적중요조성부분。
Objective To establish HPLC method for assay of astragaloside IV in NankeQingre Capsules. Methods The content of astragaloside IV was determined by a HPLC method. The Diamonsil C18 column(250mm×4.6mm,5μm)was used. The mobile phase was acetonitrile-water (35:66) and 10μl sample size with 205nm wavelength at 35℃. Results The linear range of astragaloside IV was 2.51~12.55μg (r=0.9990). The average recovery was 97.48% (n=6). Conclusion The method is simple and quick. It can be used as determination method of astragaloside IV Nanke Qingre Capsules.
