中国民康医学
中國民康醫學
중국민강의학
MEDICAL JOURNAL OF CHINSEE PEOPLE HEALTH
2014年
5期
5-7,8
,共4页
大肠癌%抑癌基因%PTEN%Syk%免疫组织化学法
大腸癌%抑癌基因%PTEN%Syk%免疫組織化學法
대장암%억암기인%PTEN%Syk%면역조직화학법
Colorectal cancer%Anti-oncogene%PTEN%Syk%Immunohistochemical method
目的：检测和分析PTEN和Syk蛋白在人大肠癌组织中的表达情况,探讨二者与大肠癌的发生、发展、浸润和转移间的关系。方法：采用免疫组织化学SP法检测60例人大肠癌组织及30例人正常大肠组织中PTEN和Syk的表达,分析二者与大肠癌患者临床病理特征之间的关系。结果：PTEN和Syk在人正常大肠组织中的表达阳性率分别为90%和96.67%,而在人大肠癌组织中的阳性率为36.67%和31.67%,差异有统计学意义(P<0.01)。两种蛋白的表达率均与患者的年龄、性别、肿瘤大小和发生部位无关(P>0.01),但与大肠癌的浸润程度、淋巴结转移、肿瘤分化程度及临床分期负相关(P<0.01)。人大肠癌中PTEN和Syk的阳性表达呈正相关(r=0.634,P<0.01)。结论：PTEN和Syk在人大肠癌组织中的表达与其发生、发展及浸润转移有密切关系,联合检测可作为判断人结直肠癌生物学行为的重要指标。
目的：檢測和分析PTEN和Syk蛋白在人大腸癌組織中的錶達情況,探討二者與大腸癌的髮生、髮展、浸潤和轉移間的關繫。方法：採用免疫組織化學SP法檢測60例人大腸癌組織及30例人正常大腸組織中PTEN和Syk的錶達,分析二者與大腸癌患者臨床病理特徵之間的關繫。結果：PTEN和Syk在人正常大腸組織中的錶達暘性率分彆為90%和96.67%,而在人大腸癌組織中的暘性率為36.67%和31.67%,差異有統計學意義(P<0.01)。兩種蛋白的錶達率均與患者的年齡、性彆、腫瘤大小和髮生部位無關(P>0.01),但與大腸癌的浸潤程度、淋巴結轉移、腫瘤分化程度及臨床分期負相關(P<0.01)。人大腸癌中PTEN和Syk的暘性錶達呈正相關(r=0.634,P<0.01)。結論：PTEN和Syk在人大腸癌組織中的錶達與其髮生、髮展及浸潤轉移有密切關繫,聯閤檢測可作為判斷人結直腸癌生物學行為的重要指標。
목적：검측화분석PTEN화Syk단백재인대장암조직중적표체정황,탐토이자여대장암적발생、발전、침윤화전이간적관계。방법：채용면역조직화학SP법검측60례인대장암조직급30례인정상대장조직중PTEN화Syk적표체,분석이자여대장암환자림상병리특정지간적관계。결과：PTEN화Syk재인정상대장조직중적표체양성솔분별위90%화96.67%,이재인대장암조직중적양성솔위36.67%화31.67%,차이유통계학의의(P<0.01)。량충단백적표체솔균여환자적년령、성별、종류대소화발생부위무관(P>0.01),단여대장암적침윤정도、림파결전이、종류분화정도급림상분기부상관(P<0.01)。인대장암중PTEN화Syk적양성표체정정상관(r=0.634,P<0.01)。결론：PTEN화Syk재인대장암조직중적표체여기발생、발전급침윤전이유밀절관계,연합검측가작위판단인결직장암생물학행위적중요지표。
Objective:To evaluate expression levels of PTEN and Syk in human colorectal cancer tissues and their relationships with the occurrence, development, metastasis, and prognosis of colorectal cancer. Methods:The expression levels of PTEN and Syk proteins in 60 cases with colorectal cancer and 30 health people were determined by the immunohistochemical SP method. Their corre-lations with clinic pathologic features were analyzed. Results:The positive rates of PTEN and Syk expression were 90% and 96. 67%in normal tissue, and 36. 67% and 31. 67% in the colorectal cancer tissues, respectively. The expression rates of the two types of pro-teins were significantly different between the two groups (P<0. 01). The expression levels of the two proteins in colorectal cancer tis-sues were not associated with patient age, gender, tumor size or tumor location (P>0. 01), but were significantly related with the dif-ferentiation degree of the cancer tissue, depth of invasion, lymph node metastasis, and Duke's staging(P<0. 01). The expression of PTEN and Syk showed positive correlations in colorectal cancer(r=0. 634, P<0. 01). Conclusions: The expression levels of PTEN and Syk in colorectal cancer are related to carcinogenesis, development, invasion, and metastasis. The joint detection of the two mark-ers can be used to determine the biological behavior and prognosis of colorectal cancer.
