CAJ | 학술논문

以螺旋藻多糖为研究对象，探讨其体内抗H22肿瘤作用及机理。采用双酶方法提取螺旋藻多糖。小鼠腋下接种H22肝癌细胞建立动物模型，以1000 mg/kg/d灌胃给药。通过瘤重及抑瘤率测定、胸腺指数（TI）及脾脏指数（SI）测定、脾细胞增殖能力测定、NK细胞杀伤活性测定、吞噬细胞吞噬能力测定、小鼠血清TNF-α和IFN-γ测定等研究其抗H22肿瘤作用和机理。给药组抑瘤率达74.53%，提高了胸腺、脾脏指数、NK细胞杀伤活性及巨噬细胞的吞噬能力，增加了血清中TNF-α和IFN-γ的含量，小鼠免疫系统得到有效保护，加强了抗H22肿瘤作用。螺旋藻多糖抗H22肿瘤效果明显，在保护免疫系统及提高免疫力方面起到了很好的作用。
이라선조다당위연구대상，탐토기체내항H22종류작용급궤리。채용쌍매방법제취라선조다당。소서액하접충H22간암세포건립동물모형，이1000 mg/kg/d관위급약。통과류중급억류솔측정、흉선지수（TI）급비장지수（SI）측정、비세포증식능력측정、NK세포살상활성측정、탄서세포탄서능력측정、소서혈청TNF-α화IFN-γ측정등연구기항H22종류작용화궤리。급약조억류솔체74.53%，제고료흉선、비장지수、NK세포살상활성급거서세포적탄서능력，증가료혈청중TNF-α화IFN-γ적함량，소서면역계통득도유효보호，가강료항H22종류작용。라선조다당항H22종류효과명현，재보호면역계통급제고면역력방면기도료흔호적작용。
To study the antitumor activity in vivo of Spirulina polysaccharide. Spirulina polysaccharide was extracted with double enzyme hydrolysis. H22 tumor bearing mice were established entity and treated by gavage with 1 000 mg/kg/d of the polysaccharide. The tumor weight, tumor inhibition rate, thymus index and Spleen index, lymphocyte proliferation, kill rate of NK cells, detection of phagocytic, the concentrations of TNF-αand IFN-γin mice blood serum were measured to study antitumor function in vivo. The tumor inhibition rate was 74.53%, The thymus index and Spleen index, lymphocyte proliferation, kill rate of NK cells were raised, and the concentrations of TNF-αand IFN-γ in mice blood serum were increased. The Spirulina polysaccharide had played an important role in protecting the immune system and promoting the immune function of tumor bearing mice.