CAJ | 학술논문

在PCR方法对肉质源性的鉴定中，大多数的研究都针对猪、牛、羊肉之间的鉴别，而对禽肉中鸡、鸭肉的鉴别研究较少。本研究在Cyt-b（细胞色素b）基因序列上确定了鸡肉和鸭肉的特异性引物，并进行了相应引物比例的PCR扩增，能够同时扩增样品中所含的鸡肉和鸭肉成分，可检测出混合样品中鸡、鸭肉的比例最低为10%。并将扩增产物进行测序比对，也进一步验证了PCR结果的准确性。该方法不仅缩短了检测时间，同时提高了检测的效率及结果的准确性，完全能够满足对市场上鸡、鸭肉的成分检测。
재PCR방법대육질원성적감정중，대다수적연구도침대저、우、양육지간적감별，이대금육중계、압육적감별연구교소。본연구재Cyt-b（세포색소b）기인서렬상학정료계육화압육적특이성인물，병진행료상응인물비례적PCR확증，능구동시확증양품중소함적계육화압육성분，가검측출혼합양품중계、압육적비례최저위10%。병장확증산물진행측서비대，야진일보험증료PCR결과적준학성。해방법불부축단료검측시간，동시제고료검측적효솔급결과적준학성，완전능구만족대시장상계、압육적성분검측。
Using PCR assay for specific detection of species origin of meat, most researches focus on the cross tested with DNA of pig, cattle and sheep, but lack of research about identifying meat species of chicken and duck. The primers used in our research were designed from correlative Cytochrome-b gene sequences, and the different proportion of primers were mixed for Multiplex PCR analysis, which can amplify the sample contained both chicken and duck in meat. Thus, the detection limits was found to be approximately 10%. Finally, the PCR products were sequenced, which could enhanced the accuracy of the result. This method not only faster than common PCR, but also increasing the accuracy and efficiency. So that this method can cover nearly all the chicken and duck in public market for species identification.