CAJ | 학술논문

建立脐橙中4种黄酮类功效成分及2种柠檬苦素类功效成分的RP-HPLC检测方法，同时测定其在脐橙中的含量。采用Agilent Eclipse XDB C18色谱柱（4.6×250 mm，5μm），流动相：梯度洗脱，A相：0.1%磷酸水；B相：乙腈，洗脱程序：23%B等度（0~5 min）；23%~55%B线性变换（5 min~10 min）；55%B等度（10 min~25 min）；检测波长：波长切换技术，0~13 min：285 nm；13 min~25 min 210 nm；柱温：30℃；流速：1.0 mL/min。结果表明柚皮苷、橙皮苷、柚皮素、橙皮素、柠檬苦素、诺米林在1.7μg/mL~266.8μg/mL范围内线性关系良好，R2均≥0.9993，6种功效成分的平均加标回收率为94.6%~111.9%。建立的方法准确、可靠，可用于该6种功效成分在脐橙样品的含量测定。
건립제등중4충황동류공효성분급2충저몽고소류공효성분적RP-HPLC검측방법，동시측정기재제등중적함량。채용Agilent Eclipse XDB C18색보주（4.6×250 mm，5μm），류동상：제도세탈，A상：0.1%린산수；B상：을정，세탈정서：23%B등도（0~5 min）；23%~55%B선성변환（5 min~10 min）；55%B등도（10 min~25 min）；검측파장：파장절환기술，0~13 min：285 nm；13 min~25 min 210 nm；주온：30℃；류속：1.0 mL/min。결과표명유피감、등피감、유피소、등피소、저몽고소、낙미림재1.7μg/mL~266.8μg/mL범위내선성관계량호，R2균≥0.9993，6충공효성분적평균가표회수솔위94.6%~111.9%。건립적방법준학、가고，가용우해6충공효성분재제등양품적함량측정。
To develop a novel quantitative method for simultaneous analysis of 6 functional components (Hesperidin, Naringin, Naringenin, Hesperetin, Limonin and Nomilin ) in navel orange by reversed-phase high performance liquid chromatography. The separation was performed on a Agilent Eclipse XDB C18 (4.6×250 mm, 5μm) and the column temperature was set at 30℃. The mobile phase was consisted of 0.1%phosphoric acid (A) and methanol (B), with a linear gradient elution at a flow rate of 1.0 mL/min. The gradient program was as flows:23%B isocratic (0-5 min);23%-55%B linear (5 min-10 min);55%B isocratic (10 min-25 min). And detection wavelength was 285 nm (0-13 min) and 210 nm(13 min-25 min). The correlation coefficients of the 6 calibration curves were all higher than 0.999 3. The scopes of the recovery rate was within the range of 94.6%-111.9 %, with RSDs no more than 2 %. The method is accurate and reliable, and can successfully analyze different navel orange samples.