CAJ | 학술논문

试验参照GenBank登录的微小牛蜱Bm91基因序列，设计了可以扩增Bm91基因完整阅读框的引物，对新疆南疆微小牛蜱进行总RNA提取、RT-PCR、克隆、测序和序列分析。结果表明：获得的Bm91基因长1833 bp，与GenBank中登录号为U62809和HM622263的微小牛蜱Bm91基因核苷酸同源性为98%和97%，且为一个完整的开放阅读框。新疆南疆微小牛蜱Bm91基因的正确获得，为基因工程疫苗候选抗原的获得和中国抗微小牛蜱疫苗的制备奠定基础。
시험삼조GenBank등록적미소우비Bm91기인서렬，설계료가이확증Bm91기인완정열독광적인물，대신강남강미소우비진행총RNA제취、RT-PCR、극륭、측서화서렬분석。결과표명：획득적Bm91기인장1833 bp，여GenBank중등록호위U62809화HM622263적미소우비Bm91기인핵감산동원성위98%화97%，차위일개완정적개방열독광。신강남강미소우비Bm91기인적정학획득，위기인공정역묘후선항원적획득화중국항미소우비역묘적제비전정기출。
In order to clone and identify the Bm91 gene of Boophilus microplus from southern Xinjiang province, the primers that can amplify complete open reading frame of Bm91 gene were designed by reference to the sequences of Bm91 gene of Boophilus microplus in GenBank. The total RNA was abstracted from Boophilus microplus from southern Xinjiang province, and then RT-PCR, cloning, sequencing and sequence analysis were utilized to it. Results showed that the obtained Bm91 gene was 1 833 bp in long;the homology between the obtained Bm91 gene and the data of registry numbers U62809 and HM622263 respectively in GenBank was 98%and 97%, respectively;the cloned Bm91 gene sequence was a complete open reading frame. The Bm91 gene of Boophilus microplus from southern Xinjiang province was obtained successfully, which lays the foundation for acquisition of candidate antigen of engineering vaccine and preparation of vaccine against Boophilus microplus in China.