中国饲料
中國飼料
중국사료
CHINA FEED
2014年
6期
27-30
,共4页
低聚壳聚糖%中华绒螯蟹%血液免疫指标%免疫保护率
低聚殼聚糖%中華絨螯蟹%血液免疫指標%免疫保護率
저취각취당%중화융오해%혈액면역지표%면역보호솔
chitooligosaccharide%Eriocheir sinensis%immune index of haemolymph%immunoprotection rate
为探讨低聚壳聚糖对中华绒螯蟹免疫功能的影响,在基础饲料中分别添加0.05‰、0.1‰、0.2‰、0.4‰的低聚壳聚糖,对均重为(15.78±3.21)g 的中华绒螯蟹进行为期28 d的饲养试验。在试验开始后第14、28天进行血液免疫学指标测定,结果表明,饲料中添加一定水平的低聚壳聚糖能够显著提高河蟹呼吸爆发活性,血清酚氧化酶、超氧化物歧化酶、溶菌酶和过氧化氢酶的活性(P<0.05)。河蟹的免疫保护率明显提高,各添加组的免疫保护率分别为12.5%、37.5%、62.5%、50%,其中200 mg添加组最高。综合考虑,低聚壳聚糖的适宜添加量为0.2‰。
為探討低聚殼聚糖對中華絨螯蟹免疫功能的影響,在基礎飼料中分彆添加0.05‰、0.1‰、0.2‰、0.4‰的低聚殼聚糖,對均重為(15.78±3.21)g 的中華絨螯蟹進行為期28 d的飼養試驗。在試驗開始後第14、28天進行血液免疫學指標測定,結果錶明,飼料中添加一定水平的低聚殼聚糖能夠顯著提高河蟹呼吸爆髮活性,血清酚氧化酶、超氧化物歧化酶、溶菌酶和過氧化氫酶的活性(P<0.05)。河蟹的免疫保護率明顯提高,各添加組的免疫保護率分彆為12.5%、37.5%、62.5%、50%,其中200 mg添加組最高。綜閤攷慮,低聚殼聚糖的適宜添加量為0.2‰。
위탐토저취각취당대중화융오해면역공능적영향,재기출사료중분별첨가0.05‰、0.1‰、0.2‰、0.4‰적저취각취당,대균중위(15.78±3.21)g 적중화융오해진행위기28 d적사양시험。재시험개시후제14、28천진행혈액면역학지표측정,결과표명,사료중첨가일정수평적저취각취당능구현저제고하해호흡폭발활성,혈청분양화매、초양화물기화매、용균매화과양화경매적활성(P<0.05)。하해적면역보호솔명현제고,각첨가조적면역보호솔분별위12.5%、37.5%、62.5%、50%,기중200 mg첨가조최고。종합고필,저취각취당적괄의첨가량위0.2‰。
A 28-day feeding trial was conducted to study the effect of diets containing different level of chi-tooligosaccharide(0‰,0.05‰,0.1‰,0.2‰,0.4‰)on immune function of Chinese mitten-handed crabs(Eriocheir sinen-sis) with initial mean weight of (15.78 ± 3.21)g.The immune indices of haemolymph was determined after 14 and 28 days of feeding.The results showed that:Chitooligosaccharide could significantly improve the activities of respiratory burst , serum phenoloxidase,superoxide dismutase,lysozyme,and catalase (P<0.05).Consequently,the immunoprotection rate of E.sinensis was enhanced.which of each adding group were 12.5%,37.5%,62.5%,50%,respectively,and 200 mg adding group was the nighest.The suitable dosage of chitooligosaccharide was 0.2‰.