CAJ | 학술논문

将240尾体重100 g左右的草鱼随机分成4组，每组4个重复，在基础日粮中添加不同水平（0、250、500、750 mg/kg）的葎草醇提物，研究其对草鱼消化道酶活力及消化道组织结构的影响。试验时间为4周。结果表明：日粮中添加葎草醇提物能不同程度地提高肠道蛋白酶和淀粉酶的活性，与对照组相比，4周平均肠道蛋白酶酶活， HSE250、HSE500、HSE750组分别提高8.28%（P>0.05）、37.96%（P＜0.05）、38.70%（P＜0.05）；4周平均淀粉酶酶活， HSE250、HSE500、HSE750组分别提高12.90%（P>0.05）、44.64%（P＜0.05）、42.23%（P＜0.05）。添加葎草醇提物对肠道菌群的数量及分布无显著影响，但可使肠黏膜的绒毛高度增高，绒毛数量增加，并降低了绒毛的宽度和肠壁的厚度，有利于肠道对营养物质的吸收。
장240미체중100 g좌우적초어수궤분성4조，매조4개중복，재기출일량중첨가불동수평（0、250、500、750 mg/kg）적률초순제물，연구기대초어소화도매활력급소화도조직결구적영향。시험시간위4주。결과표명：일량중첨가률초순제물능불동정도지제고장도단백매화정분매적활성，여대조조상비，4주평균장도단백매매활， HSE250、HSE500、HSE750조분별제고8.28%（P>0.05）、37.96%（P＜0.05）、38.70%（P＜0.05）；4주평균정분매매활， HSE250、HSE500、HSE750조분별제고12.90%（P>0.05）、44.64%（P＜0.05）、42.23%（P＜0.05）。첨가률초순제물대장도균군적수량급분포무현저영향，단가사장점막적융모고도증고，융모수량증가，병강저료융모적관도화장벽적후도，유리우장도대영양물질적흡수。
The experiment was conducted to evaluate the effects of Humulus scandens extracts (HSE) on enzyme ac-tivity and tissue morphology of intestine tract of grass carp. A total of 240 grass carp were randomly allocated to 4 dietary treatments, each treatment added 0,250,500,750 mg/kg HSE respectively,the period of experiment was 4 weeks.The re-sults showed that:HSE could increase amylase and proteases activity. Compared with the control group, intestinal protease activity of HSE250,HSE500,HSE750 group increased by 8.28%(P > 0.05),37.96%(P < 0.05),38.70%(P < 0.05),re-spectively. Compared with the control group, amylase activity of HSE250 ,HSE500,HSE750 group increased by 12.90%(P > 0.05),44.64%(P < 0.05),42.23%(P < 0.05),respectively .HSE couldn’t influence the composition of flora,but could enhance the villus height and number of intestinal villus,reduce the width and the thickness of the intestinal wall,it was conducive to the intestinal absorption of nutrients.