健康之路
健康之路
건강지로
HEALTH
2014年
10期
19-19
,共1页
归芍明目颗粒%TLC%HPLC%栀子苷
歸芍明目顆粒%TLC%HPLC%梔子苷
귀작명목과립%TLC%HPLC%치자감
Guishao Mingmu Granules%TLC%HPLC%Geniposide
目的：建立归芍明目颗粒的质量标准.方法：采用薄层色谱法(TLC)对归芍明目颗粒中栀子、甘草、白芍和牡丹皮进行定性鉴别；采用高效液相色谱法(HPLC)测定栀子中栀子苷的含量.色谱柱为 Hypersil ODS C18(4．6×250mm,5μm),以乙腈-水(12∶88)为流动相,检测波长为238nm,流速为1．0ml/min,柱温为30℃.结果：薄层色谱鉴别方法专属性强,斑点清晰,且阴性无干扰；栀子苷在0．314~1．570μg范围内线性关系良好(R＝0．9999),平均回收率为96．97％(RSD＝1．34％).结论：本质量标准方法准确、重复性好,可有效控制归芍明目颗粒的质量.
目的：建立歸芍明目顆粒的質量標準.方法：採用薄層色譜法(TLC)對歸芍明目顆粒中梔子、甘草、白芍和牡丹皮進行定性鑒彆；採用高效液相色譜法(HPLC)測定梔子中梔子苷的含量.色譜柱為 Hypersil ODS C18(4．6×250mm,5μm),以乙腈-水(12∶88)為流動相,檢測波長為238nm,流速為1．0ml/min,柱溫為30℃.結果：薄層色譜鑒彆方法專屬性彊,斑點清晰,且陰性無榦擾；梔子苷在0．314~1．570μg範圍內線性關繫良好(R＝0．9999),平均迴收率為96．97％(RSD＝1．34％).結論：本質量標準方法準確、重複性好,可有效控製歸芍明目顆粒的質量.
목적：건립귀작명목과립적질량표준.방법：채용박층색보법(TLC)대귀작명목과립중치자、감초、백작화모단피진행정성감별；채용고효액상색보법(HPLC)측정치자중치자감적함량.색보주위 Hypersil ODS C18(4．6×250mm,5μm),이을정-수(12∶88)위류동상,검측파장위238nm,류속위1．0ml/min,주온위30℃.결과：박층색보감별방법전속성강,반점청석,차음성무간우；치자감재0．314~1．570μg범위내선성관계량호(R＝0．9999),평균회수솔위96．97％(RSD＝1．34％).결론：본질량표준방법준학、중복성호,가유효공제귀작명목과립적질량.
Objective:To establish the quality standard for Guishao Mingmu Granules.Methods:Fructus Gardenniae,Radix Glycyrrhizae,Radix Paeoniae alba and Cortex Moutan were identified by TLC;and the content of Fructus Gardenniae was determined by HPLC-ELSD.The chromatographic column was Hypersil ODS C18(4.6×250mm,5μm).The mobile phase was acetonitrile-water(12∶88).The detection wavelength was at 238nm.The flow rate was 1.0ml/min and the column temperature was at 30℃.Result:The Linearity was good,and the linearrange of Geniposide was 0.314~1.570μg (R=0. 9999 ).The average recovery was 96.97%(RSD=1.34%).Conclusion:This quality standard is accurate and reproducibly,which can be used for quality control of Guishao Mingmu Granules.
