CAJ | 학술논문

用结晶致孔法制备了内嵌SiO2颗粒的聚甲基丙烯酸羟乙酯基复合晶胶，经甲基丙烯酸二甲氨乙酯接枝修饰后，得到了阴离子交换复合晶胶介质，用于从啤酒酵母转化液中色谱分离三磷酸胞苷。色谱分离过程以0.01 mol·L-1 HCl为平衡液，以0.02 mol·L-1和0.1 mol·L-1的NaCl溶液（用平衡液配制）分步洗脱。通过高效液相色谱定量分析，所得三磷酸胞苷的纯度达97.2%，回收率82.5%。与其他分离方法相比，本法过程简单、分离迅速高效，在核苷酸药物的分离领域具有很好的应用前景。
용결정치공법제비료내감SiO2과립적취갑기병희산간을지기복합정효，경갑기병희산이갑안을지접지수식후，득도료음리자교환복합정효개질，용우종비주효모전화액중색보분리삼린산포감。색보분리과정이0.01 mol·L-1 HCl위평형액，이0.02 mol·L-1화0.1 mol·L-1적NaCl용액（용평형액배제）분보세탈。통과고효액상색보정량분석，소득삼린산포감적순도체97.2%，회수솔82.5%。여기타분리방법상비，본법과정간단、분리신속고효，재핵감산약물적분리영역구유흔호적응용전경。
An anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO2 nanoparticles was prepared by cryo-polymerization followed by graft polymerization with monomer N,N-dimethylaminoethyl methacrylate. This cryogel was used to isolate cytidine triphosphate fromSaccharomyces cerevisiae transformation broth. Chromatographic separation was performed with 0.01 mol·L-1 HCl as running buffer together with 0.02 mol·L-1 and 0.1 mol·L-1 NaCl solutions (prepared in running buffer) as two-step elution liquids. Cytidine triphosphate purity was analyzed by high performance liquid chromatography. Cytidine triphosphate with high purity of 97.2% and recovery of 82.5% was obtained. Compared with other conventional methods, the present separation process was simple and effective, and could be used in separation of nucleotides from microbial fermentation or transformation feedstocks.