CAJ | 학술논문

通过测量组织液中的葡萄糖浓度获得血糖信息是目前实现血糖连续检测的主要方法，中红外ATR光谱法在分析葡萄糖等生物小分子成分信息方面具有显著优势，但是如何利用中红外ATR光谱法检测皮下组织液中的葡萄糖信息仍是尚未解决的问题。在对中红外穿透深度理论分析的基础上开展了自然状态和渗透状态下人体皮下组织液葡萄糖的中红外ATR光谱检测实验。首先探究将中红外光直接入射到人体皮肤表层上，采集自然状态下人体手指皮下组织液葡萄糖的ATR光谱数据，讨论中红外直接穿透皮下组织获得葡萄糖信息的可行性；在此基础上，通过采用低频超声和真空负压的方法促进组织液渗透到皮肤表层，采集其中红外ATR光谱进而分析和判断是否含有葡萄糖特异信息。由于二维相关光谱技术在红外光谱等光谱研究领域分析分子间反应和物质的吸收峰信息来源时有很高的分辨率和很好的适用性，利用二维相关光谱技术分别分析皮肤表层在自然状态和渗透状态的实验条件皮下组织体中葡萄糖信息。实验结果表明：中红外ATR光谱法无法直接应用于皮下组织液的葡萄糖浓度检测，利用物理或化学的方法促进组织液渗透到皮肤表层，是实现中红外ATR光谱法检测皮下组织液中葡萄糖的一个发展方向。
통과측량조직액중적포도당농도획득혈당신식시목전실현혈당련속검측적주요방법，중홍외ATR광보법재분석포도당등생물소분자성분신식방면구유현저우세，단시여하이용중홍외ATR광보법검측피하조직액중적포도당신식잉시상미해결적문제。재대중홍외천투심도이론분석적기출상개전료자연상태화삼투상태하인체피하조직액포도당적중홍외ATR광보검측실험。수선탐구장중홍외광직접입사도인체피부표층상，채집자연상태하인체수지피하조직액포도당적ATR광보수거，토론중홍외직접천투피하조직획득포도당신식적가행성；재차기출상，통과채용저빈초성화진공부압적방법촉진조직액삼투도피부표층，채집기중홍외ATR광보진이분석화판단시부함유포도당특이신식。유우이유상관광보기술재홍외광보등광보연구영역분석분자간반응화물질적흡수봉신식래원시유흔고적분변솔화흔호적괄용성，이용이유상관광보기술분별분석피부표층재자연상태화삼투상태적실험조건피하조직체중포도당신식。실험결과표명：중홍외ATR광보법무법직접응용우피하조직액적포도당농도검측，이용물리혹화학적방법촉진조직액삼투도피부표층，시실현중홍외ATR광보법검측피하조직액중포도당적일개발전방향。
Measuring the glucose concentrations in the interstitial fluid is currently the main method to achieve the continuous blood glucose monitoring .The MIR-ATR(Mid-infrared ,Attenuated Total Reflection)Spectroscopy has prominent advantage on the analysis of small biological molecule for composition information like the glucose ,but it is still an unresolved problem that how to detect the subcutaneous glucose concentration by using the MIR-ATR Spectroscopy .In the present paper ,we carry out the experiment based on MIR-ATR for the detection of subcutaneous glucose information on both the natural state and the pene-tration state based on the theoryanalysis of MIR penetration depth .Firstly ,collect spectral data of the subcutaneous glucose con-centration of human finger on the natural state were collected as the light shined the skin directly ,and it was discussed whether the MIR can penetrate the skin to get the information of subcutaneous glucose .On this basis ,collect spectral data of the subcu-taneous glucose concentration of human finger at the penetration state were collected when the Interstitial fluid is permeated to the surface layer by using low-frequency ultrasound and vacuum ,then it analyzed that whether it can detect the glucose-specific information or not .As the two-dimensional correlation spectroscopy has high resolution and good versatility ,it is widely used to analyze the inter-molecular reaction and judge the absorption peaks information in many fields including the M IR spectroscopy field ,so we choose the Two-dimensional correlation spectroscopy to analyze the information of subcutaneous glucose concentra-tion at the natural state and the penetration state .The experiment result shows that the MIR-ATR spectroscopy can’t be applied in the detection of subcutaneous glucose concentrationdirectly ,and it is a promising direction to make the Interstitial fluid perme-ated to the surface layer by the physical methods or chemical methods .