中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
17期
41-42,43
,共3页
巴桑卓嘎%武尉杰%周雪杉%吴梅%谭睿
巴桑卓嘎%武尉傑%週雪杉%吳梅%譚睿
파상탁알%무위걸%주설삼%오매%담예
二十味肉豆蔻丸%肉豆蔻醚%薄层色谱法%高效液相色谱法
二十味肉豆蔻汍%肉豆蔻醚%薄層色譜法%高效液相色譜法
이십미육두구환%육두구미%박층색보법%고효액상색보법
Ershiwei Roudoukou Pills%myristicin%TLC%HPLC
目的：建立藏药二十味肉豆蔻丸的质量控制方法。方法采用薄层色谱法对处方中肉豆蔻、红花、乳香、降香进行定性鉴别；采用高效液相色谱法测定肉豆蔻醚的含量，色谱柱为Comatex C18柱(250 mm ×4.6 mm，5μm )，流动相为甲醇-水(60：40)，检测波长为276 nm，柱温35℃，体积流量为1.0 mL/min。结果肉豆蔻醚质量浓度在0.1044~1.044 g/L范围内与峰面积线性关系良好( r2=0.9992)，平均回收率为101.46%，RSD为1.94%（ n=6）。结论该方法简便、准确、专属性强，可用于二十味肉豆蔻丸的质量控制。
目的：建立藏藥二十味肉豆蔻汍的質量控製方法。方法採用薄層色譜法對處方中肉豆蔻、紅花、乳香、降香進行定性鑒彆；採用高效液相色譜法測定肉豆蔻醚的含量，色譜柱為Comatex C18柱(250 mm ×4.6 mm，5μm )，流動相為甲醇-水(60：40)，檢測波長為276 nm，柱溫35℃，體積流量為1.0 mL/min。結果肉豆蔻醚質量濃度在0.1044~1.044 g/L範圍內與峰麵積線性關繫良好( r2=0.9992)，平均迴收率為101.46%，RSD為1.94%（ n=6）。結論該方法簡便、準確、專屬性彊，可用于二十味肉豆蔻汍的質量控製。
목적：건립장약이십미육두구환적질량공제방법。방법채용박층색보법대처방중육두구、홍화、유향、강향진행정성감별；채용고효액상색보법측정육두구미적함량，색보주위Comatex C18주(250 mm ×4.6 mm，5μm )，류동상위갑순-수(60：40)，검측파장위276 nm，주온35℃，체적류량위1.0 mL/min。결과육두구미질량농도재0.1044~1.044 g/L범위내여봉면적선성관계량호( r2=0.9992)，평균회수솔위101.46%，RSD위1.94%（ n=6）。결론해방법간편、준학、전속성강，가용우이십미육두구환적질량공제。
Objective To establish a quality control method for Tibetan drug Ershiwei Roudoukou Pills. Methods Semen myristicae, Carthamus tinctorious, Olibanum, Lignum dalbergiae odoriferae were qualitatively identify by TLC;the contents of myristicin in Myristicae semen were determined by HPLC. The chromatographic column was the Comatex C18 column ( 250 mm × 4. 6 mm, 5μm ) with methanol-water ( 60:40 ) as the mobile phase. The UV detection wavelength was set at 276 nm and the column temperature was 35 ℃ with the flow rate of 1. 0 mL/min. Results Myristicin within the range of 0. 104 4-1. 044 mg/mL showed a good linear relationship ( r2=0. 999 2 ) , the average recovery rate was 101. 46%, RSD 1. 94%. Conclusion The method is simple, exact and strongly specific, and can be used for the quality control of Ershiwei Roudoukou Pills.
