中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
17期
32-33,34
,共3页
贝那普利%血浆%高效液相色谱法
貝那普利%血漿%高效液相色譜法
패나보리%혈장%고효액상색보법
benazepril%plasma%HPLC
目的：建立测定人血浆中贝那普利浓度的反相高效液相色谱（HPLC）法。方法采用液-液萃取法分离纯化血浆样品，采用荧光检测器，检测波长为240 nm，以pH=2.2的0.02 mol/L（含3.0%三乙胺）磷酸盐缓冲液-甲醇（37：63）为流动相，色谱柱为Shim-Pack CLC-ODS柱（150 mm ×4.6 mm，3.5μm）。结果贝那普利色谱峰对称、不拖尾、无其他干扰，血药浓度线性范围为0.52~60.04μg/L ( r=0.9999)，最低检测限为0.4μg/L，方法回收率为98.43%~100.68%，日内精密度 RSD 2.40%~4.20%，日间精密度 RSD 2.80%~4.80%。结论方法简便、准确，灵敏度高，选择性好，可用于贝那普利的血药浓度测定。
目的：建立測定人血漿中貝那普利濃度的反相高效液相色譜（HPLC）法。方法採用液-液萃取法分離純化血漿樣品，採用熒光檢測器，檢測波長為240 nm，以pH=2.2的0.02 mol/L（含3.0%三乙胺）燐痠鹽緩遲液-甲醇（37：63）為流動相，色譜柱為Shim-Pack CLC-ODS柱（150 mm ×4.6 mm，3.5μm）。結果貝那普利色譜峰對稱、不拖尾、無其他榦擾，血藥濃度線性範圍為0.52~60.04μg/L ( r=0.9999)，最低檢測限為0.4μg/L，方法迴收率為98.43%~100.68%，日內精密度 RSD 2.40%~4.20%，日間精密度 RSD 2.80%~4.80%。結論方法簡便、準確，靈敏度高，選擇性好，可用于貝那普利的血藥濃度測定。
목적：건립측정인혈장중패나보리농도적반상고효액상색보（HPLC）법。방법채용액-액췌취법분리순화혈장양품，채용형광검측기，검측파장위240 nm，이pH=2.2적0.02 mol/L（함3.0%삼을알）린산염완충액-갑순（37：63）위류동상，색보주위Shim-Pack CLC-ODS주（150 mm ×4.6 mm，3.5μm）。결과패나보리색보봉대칭、불타미、무기타간우，혈약농도선성범위위0.52~60.04μg/L ( r=0.9999)，최저검측한위0.4μg/L，방법회수솔위98.43%~100.68%，일내정밀도 RSD 2.40%~4.20%，일간정밀도 RSD 2.80%~4.80%。결론방법간편、준학，령민도고，선택성호，가용우패나보리적혈약농도측정。
Objective To develop a HPLC method for quantifying the benazepril concentration in human plasma. Methods Sample plas-ma was isolated by the liquid-liquid extraction method and the UV detector was adopted with the detection wavelength of 240 nm. The mobile phase was phosphate buffer solution ( pH=2. 2, 0. 02 mol/L ) containing 3. 0% triethylamine-methanol ( 37 : 63 ); the chromato-graphic column was the Shim-Pack CLC-ODS column ( 150 mm × 4. 6 mm, 3. 5 μm ) . Results The chromatographic peak of benazepril was symmetric and non-trailing without other interference. The linear range of plasma benazepril concentration was 0. 52-60. 04μg/L ( r=0. 999 9 ) . The lowest detection limit of benazepril in plasma was 0. 4μg/L. The recovery rate was 98. 43% -100. 68%. The intra-day and inter-day RSD were 2. 40% -4. 20% and 2. 80% -4. 80% respectively. Conclusion The established method is simple, ac-curate, and highly sensitive, and can be used for detecting the benazepril concentration in plasma.
